CAJ | 학술논문

以阿月浑子良种‘科尔曼'的种子和枝条作为外植体进行组织培养.结果表明:1/2 DKW和1/2 DKW+6-BA 3.0mg/L+NAA0.05 mg/L作为种子萌发和茎段分化的培养基;1/2 DKW+6-BA 4.0 mg/L+IBA 0.05 mg/L为增殖培养基,增殖系数可达3.6;1/2 DKW+IBA 5 mg/L+NAA 1 mg/L为生根培养基,生根率75%以上,移栽在河砂与蛭石(3:1)混合基质中,成活率达79%以上.
이아월혼자량충‘과이만'적충자화지조작위외식체진행조직배양.결과표명:1/2 DKW화1/2 DKW+6-BA 3.0mg/L+NAA0.05 mg/L작위충자맹발화경단분화적배양기;1/2 DKW+6-BA 4.0 mg/L+IBA 0.05 mg/L위증식배양기,증식계수가체3.6;1/2 DKW+IBA 5 mg/L+NAA 1 mg/L위생근배양기,생근솔75%이상,이재재하사여질석(3:1)혼합기질중,성활솔체79%이상.
[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement.[Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ' Kerman',tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW +3.00 mg/L of 6-BA +0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio;Proliferation coefficient was 3.6 on the medium of 1/2DKW +4.00 mg/L of 6-BA + 0.05 mg/L of IBA;Rooting rate was up to 75% on the medium of 1/2DKW + 5.00 mg/L of IBA+ 1.00 mg/L of NAA;The suitable transplantation matrix was 3/4 sand + 1/4 vermiculite,and survival rate of plant with root was above 79%.[Conclusion]The pistachio (Kerman) tissue culture technology system was established.