CAJ | 학술논문

目的研究不同分割剂量电离辐射方案对卵巢癌细胞多药耐药性的影响.方法采用卵巢癌亲本细胞SKOV3及其耐药细胞株SKVCR,分别进行假照射、单次照射(10 Gy)、常规分割照射(2 Gy ×5)和超分割照射(1 Gy×2 ×5).MTT方法检测细胞对4种化疗药物硫酸长春新碱( vincristine,VCR)、依托泊苷(etoposide,VP-16)、盐酸吡柔比星(pirarubicin,THP)和顺铂(cisplatin,DDP)敏感性的变化.Western blot检测P-gp蛋白表达量.结果与SKOV3相比,SKVCR倍增时间增加为1.8倍,P-gp糖蛋白表达增高,4种药物对SKVCR的IC50浓度明显增加(P＜0.05).在SKOV3中,与假照组相比,单次照射后细胞对THP、DDP药敏性降低,常规分割照射后细胞对VCR、THP、VP-16药敏性降低,超分割照射使VP-16药敏性降低；在SKVCR中,与假照组相比,3个照射组对VCR、VP-16的药敏性增高,对DDP的药敏性无明显改变,单次和分割照射均可降低P-gp表达.结论单次、分割和超分割照射在SKOV3亲本细胞中诱导多药耐药,在耐药株SKVCR中逆转对VCR、VP-16的耐药性.
목적 연구불동분할제량전리복사방안대란소암세포다약내약성적영향.방법 채용란소암친본세포SKOV3급기내약세포주SKVCR,분별진행가조사、단차조사(10 Gy)、상규분할조사(2 Gy ×5)화초분할조사(1 Gy×2 ×5).MTT방법검측세포대4충화료약물류산장춘신감( vincristine,VCR)、의탁박감(etoposide,VP-16)、염산필유비성(pirarubicin,THP)화순박(cisplatin,DDP)민감성적변화.Western blot검측P-gp단백표체량.결과 여SKOV3상비,SKVCR배증시간증가위1.8배,P-gp당단백표체증고,4충약물대SKVCR적IC50농도명현증가(P＜0.05).재SKOV3중,여가조조상비,단차조사후세포대THP、DDP약민성강저,상규분할조사후세포대VCR、THP、VP-16약민성강저,초분할조사사VP-16약민성강저；재SKVCR중,여가조조상비,3개조사조대VCR、VP-16적약민성증고,대DDP적약민성무명현개변,단차화분할조사균가강저P-gp표체.결론 단차、분할화초분할조사재SKOV3친본세포중유도다약내약,재내약주SKVCR중역전대VCR、VP-16적내약성.
Objective To investigate the effect of different subtypes of fractionated doses on multidrug resistance in ovarian cancer cells.Methods The human ovarian cancer cell lines SKOV3 and its drug-resistant subtype SKVCR were divided into four groups i.e., sham-irradiated, single dose (10 Gy),fractionated dose (2 Gy × 5 ) and multi-fractionated dose (1 Gy × 2 × 5).Cell sensitivity to vincristine(VCR),etoposide ( VP-16),pirarubicin (THP) and cisplatin (DDP) was measured by MTT assay.Western blot was applied to detect the expression of P-gp after irradiation.Results The doubling time of SKVCR was about 1.8-fold of that of SKOV3 cells.P-gp was expressed in SKVCR but not in SKOV3.IC50 values of SKVCR were higher than those of SKOV3.To SKOV3 cells,single dose irradiation decreased cell sensitivity to THP and DDP and fractionated irradiation decreased cell sensitivity to VCR,THP and VP-16.Multi-fractionated irradiation decreased cell sensitivity to VP-16.In SKVCR cells,all these irradiation treatments increased cell sensitivity to VCR and VP-16 but not to DDP.In addition,single and fractionated irradiation decreased P-gp expression in SKVCR cells.Conclusions Single,fractionated and multi-fractionated radiation induced chemotherapy resistance in SKOV3 cells,while reversed drug resistance to VCR and VP-16 in SKVCR cells.