CAJ | 학술논문

目的研究自然杀伤(NK)细胞对多发性骨髓瘤细胞株KM-3杀伤及诱导凋亡的作用.方法 WST-1法观察不同效靶比的NK细胞对KM-3细胞的杀伤作用;流式细胞术检测Annexin-V+/PI-凋亡细胞、线粒体跨膜电位.结果 NK细胞作用于KM-3细胞后,能显著杀伤KM-3细胞,呈剂量和时间依赖性(P<0.05);NK细胞作用于KM-3细胞48 h后,Annexin-V+/PI-细胞比例明显增加,呈剂量依赖性(P<0.05);NK细胞作用于KM-3细胞效靶比为10:1时,Annexin-V+/PI-细胞比例明显增加,呈时间依赖性(P<0.05);NK细胞作用于KM-3细胞后,KM-3细胞线粒体跨膜电位明显降低,呈剂量和时间依赖性(P<0.05).结论 NK细胞能明显杀伤KM-3细胞并诱导其凋亡,均呈剂量和时间依赖性.
목적 연구자연살상(NK)세포대다발성골수류세포주KM-3살상급유도조망적작용.방법 WST-1법관찰불동효파비적NK세포대KM-3세포적살상작용;류식세포술검측Annexin-V+/PI-조망세포、선립체과막전위.결과 NK세포작용우KM-3세포후,능현저살상KM-3세포,정제량화시간의뢰성(P<0.05);NK세포작용우KM-3세포48 h후,Annexin-V+/PI-세포비례명현증가,정제량의뢰성(P<0.05);NK세포작용우KM-3세포효파비위10:1시,Annexin-V+/PI-세포비례명현증가,정시간의뢰성(P<0.05);NK세포작용우KM-3세포후,KM-3세포선립체과막전위명현강저,정제량화시간의뢰성(P<0.05).결론 NK세포능명현살상KM-3세포병유도기조망,균정제량화시간의뢰성.
Objective To study the apoptosis of multiple myeloma cell line KM-3 induced by NK cells. Methods WST-1 assay was used to detect the killing effect of KM-3 cells treated with NK cells at different effector(E):target(T) ratio. Flow cytometry was applied to analyze Annexin-V+/PI- apoptotic cells and the mitochondrial transmembrane potential. Results NK cells could significantly kill KM-3 cells in a dosand time-dependent manner (P <0.05). After KM-3 cells- were treated with NK cells for 48 hours, the Annexin-V+/PI- cells were increased obviously in dose-dependence (P <0.05). The Annexin-V+PI- cells were increased in time-dependence when treated with NK cells(E:T ratio at 10:1) (P<0.05). The mitochondrial transmembrane potential of KM-3 cells treated with NK cells were significantly decreased in dose-and time-dependence (P < 0.05). Conclusion NK cell can kill KM-3 cells and induce apoptosis in a dose-and time-dependence manner.