中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2008年
11期
1123-1127
,共5页
吴庆刚%张敬平%陈锦英%郑明寰%尤凤兴%姜永
吳慶剛%張敬平%陳錦英%鄭明寰%尤鳳興%薑永
오경강%장경평%진금영%정명환%우봉흥%강영
尿道致病性大肠埃希菌%基因测序%未知基因型
尿道緻病性大腸埃希菌%基因測序%未知基因型
뇨도치병성대장애희균%기인측서%미지기인형
Uropathogenic Escherichia coli%Gene sequencing%Unkown genotype
目的 比较尿道致病性大肠埃希菌UPECA030株papG和papA基因与相关序列的差异.以明确UPECA030 papA基因变异情况及与papG的组合形式,确定是否为未知基因型.方法 采用基因克隆测序方法,分别对UPEC4030株papG和papA基因进行序列分析,并与相关序列进行比较.结果 UPECA030 papA由722个碱基对组成,编码192个氨基酸的多肽.与10个基因型UPEC papA核苷酸序列和推导的氨基酸序列同源性比较,分别为36.11%～77.95%和22.20%～78.34%;与papA基因分型多重PCR引物序列比较,下游分型引物在UPEC4030 papa相应位置上的序列同源性只有10.00%～66.67%,表明该菌株papA确实为未知的基因型.UPECA030菌株papG全基因片段由1100个碱基组成,编码337个氨基酸的多肽,其核苷酸及推导的氨基酸序列与Ⅱ型抛papG代表株UPEC IA2相比较,同源性分别为99.00%和99.11%,表明UPEC4030 papA属于Ⅱ型.结论 UPECA030 papA为未知的摹因型,其papG属于Ⅱ型.
目的 比較尿道緻病性大腸埃希菌UPECA030株papG和papA基因與相關序列的差異.以明確UPECA030 papA基因變異情況及與papG的組閤形式,確定是否為未知基因型.方法 採用基因剋隆測序方法,分彆對UPEC4030株papG和papA基因進行序列分析,併與相關序列進行比較.結果 UPECA030 papA由722箇堿基對組成,編碼192箇氨基痠的多肽.與10箇基因型UPEC papA覈苷痠序列和推導的氨基痠序列同源性比較,分彆為36.11%～77.95%和22.20%～78.34%;與papA基因分型多重PCR引物序列比較,下遊分型引物在UPEC4030 papa相應位置上的序列同源性隻有10.00%～66.67%,錶明該菌株papA確實為未知的基因型.UPECA030菌株papG全基因片段由1100箇堿基組成,編碼337箇氨基痠的多肽,其覈苷痠及推導的氨基痠序列與Ⅱ型拋papG代錶株UPEC IA2相比較,同源性分彆為99.00%和99.11%,錶明UPEC4030 papA屬于Ⅱ型.結論 UPECA030 papA為未知的摹因型,其papG屬于Ⅱ型.
목적 비교뇨도치병성대장애희균UPECA030주papG화papA기인여상관서렬적차이.이명학UPECA030 papA기인변이정황급여papG적조합형식,학정시부위미지기인형.방법 채용기인극륭측서방법,분별대UPEC4030주papG화papA기인진행서렬분석,병여상관서렬진행비교.결과 UPECA030 papA유722개감기대조성,편마192개안기산적다태.여10개기인형UPEC papA핵감산서렬화추도적안기산서렬동원성비교,분별위36.11%～77.95%화22.20%～78.34%;여papA기인분형다중PCR인물서렬비교,하유분형인물재UPEC4030 papa상응위치상적서렬동원성지유10.00%～66.67%,표명해균주papA학실위미지적기인형.UPECA030균주papG전기인편단유1100개감기조성,편마337개안기산적다태,기핵감산급추도적안기산서렬여Ⅱ형포papG대표주UPEC IA2상비교,동원성분별위99.00%화99.11%,표명UPEC4030 papA속우Ⅱ형.결론 UPECA030 papA위미지적모인형,기papG속우Ⅱ형.
Objective To investigate the differences between the sequences of papA and papG of UPECA030 strain and the related genes, to better understand the genetic variation of UPEC4030 papA and its combination forms with papG so as to identify if it was a new genotype. Methods Cloning and sequencing methods were used to analyze the sequences of papG and papA of UPEC4030 strain and to compare their related sequences. Results Through sequence analysis of papA, it was revealed that there was a 722 bp gene,encoding 192 amino acid polypeptide. The overall homology of the papa genes between UPEC4030 and the standard strains of ten F types were 36.11%-77.95 % and 22.20%-78.34% at nueleotide and deduced amino acid levels. Homology between the sequences of reverse primers and the corresponding sequence of UPEC4030 papA was 10.00%-66.67%. The results confirmed that UPECA030 strain contained a novel papA variant. Through sequence analysis of UPEC4030 papG, we revealed a 1100 bp gene, encoding 337 amino acid polypeptide. The homology of the papG genes between UPEC4030 and UPEC IA2, the standard strain, was 99.00 % at nucleotide level and 99.11% at deduced amino acid and UPEC4030 strain carried class I] genotype of papG. Conclusion UPEC4030 strain contained an unknown papA variant or the new genotype and carried class II genotype of papG. The pathogenic mechanism and epidemiology call for further study.
