CAJ | 학술논문

目的探讨三磷酸腺苷结合盒转运体成员2(ABCG2)在人脑胶质瘤组织中的表达及其与胶质瘤分级和神经巢蛋白(nestin)表达的关系.方法实时荧光定量聚合酶链反应(PCR)检测ABCG2在52例不同病理级别胶质瘤中的mRNA表达;免疫组织化学SABC法检测ABCG2和nestin的蛋白表达.结果 ABCG2在脑胶质瘤中的mRNA表达与正常脑组织比较呈过表达(P<0.05),且随着病理级别的升高而增加(P<0.05);免疫组织化学显示ABCG2在52例胶质瘤中的阳性表达率为32.7%(17/52),并与病理级别明显相关(x2=4.62,P<0.05),主要呈亲血管分布;ABCG2的表达与nestin的表达明显相关(x2=7.60,P<0.05).结论 ABCG2在人脑胶质瘤中的表达随着病理级别的升高而逐渐增加,且与nestin的表达呈正相关;脑肿瘤干细胞可能与神经干细胞具有一定的同源性.
목적 탐토삼린산선감결합합전운체성원2(ABCG2)재인뇌효질류조직중적표체급기여효질류분급화신경소단백(nestin)표체적관계.방법 실시형광정량취합매련반응(PCR)검측ABCG2재52례불동병리급별효질류중적mRNA표체;면역조직화학SABC법검측ABCG2화nestin적단백표체.결과 ABCG2재뇌효질류중적mRNA표체여정상뇌조직비교정과표체(P<0.05),차수착병리급별적승고이증가(P<0.05);면역조직화학현시ABCG2재52례효질류중적양성표체솔위32.7%(17/52),병여병리급별명현상관(x2=4.62,P<0.05),주요정친혈관분포;ABCG2적표체여nestin적표체명현상관(x2=7.60,P<0.05).결론 ABCG2재인뇌효질류중적표체수착병리급별적승고이축점증가,차여nestin적표체정정상관;뇌종류간세포가능여신경간세포구유일정적동원성.
Objective To study the expression of ATP-binding cassette superfamily G member 2 ( ABCG2) and its relationship with the malignant degree and expression of nestin in human gliomas. Methods Fifty-two gliomas of different WHO grades and 8 normal brain tissues were detected for the mRNA expression of ABCG2 by using real-time quantitive polymerase chain reaction (PCR). And the protein expression of ABCG2 and nestin was detected by immunohistochemistry. Results The mRNA of ABCG2 was overexpressed in gliomas as compared with that in normal brain tissues (P<0. 05), and up-regulated with the increase of pathologic degrees (P<0. 05 ). The positive protein expression rate of ABCG2 in 52 gliomas was 32.7%. The positive rate was significantly higher in grade Ⅲ-Ⅳ than in grade Ⅰ -Ⅱ (x2 =4. 62, P < 0. 05). And the expression level of ABCG2 was obviously related with the expression of nestin (x2= 7. 60,P < 0.05). Conclusion The expression level of ABCG2 was obviously related with glioma pathological grade and the expression of nestin. Brain tumor stem cells may have some homology with nerve stem cells.