中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2008年
9期
624-627
,共4页
李凤鹏%于生元%管维平%石宏%陈淑莉%晋志高%吴士文%董钊%邱恩超%刘若卓%姜磊
李鳳鵬%于生元%管維平%石宏%陳淑莉%晉誌高%吳士文%董釗%邱恩超%劉若卓%薑磊
리봉붕%우생원%관유평%석굉%진숙리%진지고%오사문%동쇠%구은초%류약탁%강뢰
水管周灰质%三唑类%大脑皮质%原癌基因蛋白质c-fos%基因,fos
水管週灰質%三唑類%大腦皮質%原癌基因蛋白質c-fos%基因,fos
수관주회질%삼서류%대뇌피질%원암기인단백질c-fos%기인,fos
Periaqueductal gray%Triazoles%Cerebral cortex%Proto-oncogene proteins c-fos%Genes,fos
目的 观察苯甲酸利扎曲普坦对大鼠皮质扩布性抑制(cortical spreading depression,CSD)和CSD引起中脑导水管周围灰质(periaqueduetal grey,PAG)内c-Fos表达的影响.方法 实验选用的SD大鼠随机分为3组,A组:氯化钾刺激组;B组:氯化钾刺激+苯甲酸利扎曲普坦组;C组:氯化钠刺激组.对各组大鼠使用氯化钾(或氯化钠)刺激,以观察CSD出现的个数和波幅;第1次刺激2 h后使用免疫组织化学法检测各组PAG内不同层面c-Fos阳性神经元.结果 C组未记录到CSI).A组CSD个数(10.70±3.23)较B组(6.10±2.56)显著增多(t=-3.528,P<0.01);A组CSD波幅[17.33(95%CI 11.45～23.11)mV]较B组[11.82(95%CI 9.24～14.70)mV]显著增加(Z=-4.360,P<0.01).A组各层面c-Fos阳性细胞数较B、C组显著增多(P<0.05).结论 苯甲酸利扎曲普坦对CSD有抑制作用,可使CSD引起PAG内神经元兴奋作用减弱.
目的 觀察苯甲痠利扎麯普坦對大鼠皮質擴佈性抑製(cortical spreading depression,CSD)和CSD引起中腦導水管週圍灰質(periaqueduetal grey,PAG)內c-Fos錶達的影響.方法 實驗選用的SD大鼠隨機分為3組,A組:氯化鉀刺激組;B組:氯化鉀刺激+苯甲痠利扎麯普坦組;C組:氯化鈉刺激組.對各組大鼠使用氯化鉀(或氯化鈉)刺激,以觀察CSD齣現的箇數和波幅;第1次刺激2 h後使用免疫組織化學法檢測各組PAG內不同層麵c-Fos暘性神經元.結果 C組未記錄到CSI).A組CSD箇數(10.70±3.23)較B組(6.10±2.56)顯著增多(t=-3.528,P<0.01);A組CSD波幅[17.33(95%CI 11.45～23.11)mV]較B組[11.82(95%CI 9.24～14.70)mV]顯著增加(Z=-4.360,P<0.01).A組各層麵c-Fos暘性細胞數較B、C組顯著增多(P<0.05).結論 苯甲痠利扎麯普坦對CSD有抑製作用,可使CSD引起PAG內神經元興奮作用減弱.
목적 관찰분갑산리찰곡보탄대대서피질확포성억제(cortical spreading depression,CSD)화CSD인기중뇌도수관주위회질(periaqueduetal grey,PAG)내c-Fos표체적영향.방법 실험선용적SD대서수궤분위3조,A조:록화갑자격조;B조:록화갑자격+분갑산리찰곡보탄조;C조:록화납자격조.대각조대서사용록화갑(혹록화납)자격,이관찰CSD출현적개수화파폭;제1차자격2 h후사용면역조직화학법검측각조PAG내불동층면c-Fos양성신경원.결과 C조미기록도CSI).A조CSD개수(10.70±3.23)교B조(6.10±2.56)현저증다(t=-3.528,P<0.01);A조CSD파폭[17.33(95%CI 11.45～23.11)mV]교B조[11.82(95%CI 9.24～14.70)mV]현저증가(Z=-4.360,P<0.01).A조각층면c-Fos양성세포수교B、C조현저증다(P<0.05).결론 분갑산리찰곡보탄대CSD유억제작용,가사CSD인기PAG내신경원흥강작용감약.
Objective To determine whether rizatriptan has an effect on cortical spreading depression (CSD) and c-Fos expression within periaqueductal grey (PAG) induced by CSD in rats. Methods The experimental SD rats were randomly divided into group A injected with KCl, group B KCl plus rizatriptan and group C NaCL The number and amplitude of CSD were recorded after KCl or NaCl injection. C-Fos positive neurons of different layer were identified by the immunohistochemical technique 2 hours after the first injection of KCl or NaCl. Results There was no CSD in group C. The number of CSD in group A ( 10.70±3.23 ) was significantly more than that in group B (6.10±2.56, t = - 3.528, P < 0.01 ). The amplitude of CSD in group A ( 17.33 (95% CI 11.45--23.11 ) mV) was significantly greater than that in group B (11.82 (95%CI 9.24--14.70) mV, Z= -4.360, P< 0.01). There were more cFos-like immnoreactive neurons in every layer in group A than in group C (P < 0.01 ) and in group B (P < 0.05 ). Conclusion Rizatriptan has an inhibitory effect on CSD, which might induce the headache through exciting the neurons in PAG.
