CAJ | 학술논문

目的观察RNA干扰对大肠癌细胞中人端粒酶逆转录酶基因(hTERT gene)的抑制效应.方法大肠癌细胞株HT-29分为Control组、N-hTERT组、Lipofectamine组和sihTERT组;将靶向于hTERT基因的小干扰RNA,转染大肠癌细胞;分别检测大肠癌细胞转染前后的端粒酶活性,hTERTmRNA和蛋白水平,细胞生长增殖和凋亡情况.结果 sihTERT转染大肠癌细胞效率为60%;sihTERT组的端粒酶活性值、hTERT mRNA表达、hTERT蛋白表达、细胞凋亡率分别为0.73±0.14、0.47±0.08、0.37±0.07、49.5%,sihTERT组与其他三组比较差异有统计学意义(P<0.01).结论靶向于hTERT的siRNA能特异性沉默大肠癌细胞hTERT基因,下调hTERT基因mRNA及蛋白表达水平,降低端粒酶活性,抑制癌细胞生长增殖从而促进大肠癌细胞凋亡.
목적 관찰RNA간우대대장암세포중인단립매역전록매기인(hTERT gene)적억제효응.방법 대장암세포주HT-29분위Control조、N-hTERT조、Lipofectamine조화sihTERT조;장파향우hTERT기인적소간우RNA,전염대장암세포;분별검측대장암세포전염전후적단립매활성,hTERTmRNA화단백수평,세포생장증식화조망정황.결과 sihTERT전염대장암세포효솔위60%;sihTERT조적단립매활성치、hTERT mRNA표체、hTERT단백표체、세포조망솔분별위0.73±0.14、0.47±0.08、0.37±0.07、49.5%,sihTERT조여기타삼조비교차이유통계학의의(P<0.01).결론 파향우hTERT적siRNA능특이성침묵대장암세포hTERT기인,하조hTERT기인mRNA급단백표체수평,강저단립매활성,억제암세포생장증식종이촉진대장암세포조망.
Objective To explore the inhibitory effect of RNA interference on the human telomer-ase reverse transcriptase gene in colon cancer cells. Methods Colon cancer HT-29 cells were divided into control group,N-hTERT group, Lipofectamine group,and sihTERT group. The activity of telomerase,and expression of hTERT mRNA and protein were detected before and after siRNA was transfected into HT-29 cells. Flow cytometry was used to observe the proliferation and apoptosis of HT-29 cells. Results The transfection efficiency of siRNA was 60%. The activity of telomerase, the expression levels of hTERT mR-NA and protein, and apeptosis rate in sihTERT group were 0.73±0.14,0.47±0.08,0.37±0.07, and 49.5% respectively,which were significantly different from those in other three groups (P<0.01). Con-clusion siRNA targeting hTERT can specificly scilence the hTERT gene of colon cancer cells by down-regulating the expression of hTERT mRNA and protein, and suppressing the telomerase activity. RNAi can inhibit the proliferation of the cancer cells and induce their apoptosis.