CAJ | 학술논문

对两种不同的蚂蚁窝进行甲醇提取,并对提取物进行了急性毒性、抗自由基,酚含量、还原能力和抗菌活性的检测.二苯基三硝基肼(1,1-diphenyl-2-picrylhydrazyl ,DPPH)自由基的清除能力的检测表明:两种蚂蚁窝甲醇提取物都具有显著的自由基清除能力,并呈现出剂量依赖性,而且,在高浓度时(5～10 mg·mL-1),它们的自由基清除能力甚至显著地高于二丁基羟基甲苯(butylated hydroxyl toluene,BHT).酚的含量和还原能力分别用福林酚试剂和铁氰化钾还原反应来测定,统计分析显示两种蚂蚁窝的抗氧化能力和其酚含量、还原能力都存在着正相关.两种蚂蚁窝甲醇提取物都具有抗革兰氏阴性菌和革兰氏阳性菌的活性,但它们却对真菌没有作用.
대량충불동적마의와진행갑순제취,병대제취물진행료급성독성、항자유기,분함량、환원능력화항균활성적검측.이분기삼초기정(1,1-diphenyl-2-picrylhydrazyl ,DPPH)자유기적청제능력적검측표명:량충마의와갑순제취물도구유현저적자유기청제능력,병정현출제량의뢰성,이차,재고농도시(5～10 mg·mL-1),타문적자유기청제능력심지현저지고우이정기간기갑분(butylated hydroxyl toluene,BHT).분적함량화환원능력분별용복림분시제화철청화갑환원반응래측정,통계분석현시량충마의와적항양화능력화기분함량、환원능력도존재착정상관.량충마의와갑순제취물도구유항혁란씨음성균화혁란씨양성균적활성,단타문각대진균몰유작용.
Methanol extracts of two kinds of formicary were investigated for their free radical scavenging activities, total phenolic content, reduction power and antimicrobial activities. Both of them showed no toxic effect on mice at the testing dose of 10 mg/kg (p.o). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging test showed that both of the formicary extracts were potent radical scavengers and their scavenging activities were dose-dependent. Furthermore, at higher concentrations (5～10 mg/ml) both of them exhibited significantly higher DPPH scavenging activity than butylated hydroxytoluene (BHT). Total phenolic content and reducing power were determined using the Folin-Ciocalteu and the potassium ferricyanide reduction methods, respectively. Statistical analysis indicated a close association between the antioxidant potency and total phenolic content, and between the antioxidant potency and reducing power as well. Both methanol extracts presented antimicrobial activities on gram-negative and gram-positive bacteria. However, they were inactive on fungus.