CAJ | 학술논문

目的探讨卵泡液中CD56~+自然杀伤(NK)细胞及活化的cD56~+NK(CD56~+CD69~+NK)细胞的比例与体外受精(IVF)结局的关系.方法应用流式细胞仪分析成熟卵泡液中CD56~+NK细胞及活化的CD56+NK细胞的比例,结合IVF治疗结果,探讨其相关性.结果妊娠患者的卵泡液中平均CD56~+NK细胞及活化的CD56~+NK细胞分别为(15.57±3.10)%和(2.63±0.94)%,未妊娠患者的卵泡液中平均CD56~+NK细胞及活化的CD56~+NK细胞分别为(19.12±5.37)%和(4.06±2.08)%,显著高于妊娠组(P<0.05).结论接受IVF助孕治疗患者中成熟卵泡液中CD56~+NK细胞及活化的CD56~+NK细胞的比例较低者易获临床妊娠.
목적 탐토란포액중CD56~+자연살상(NK)세포급활화적cD56~+NK(CD56~+CD69~+NK)세포적비례여체외수정(IVF)결국적관계.방법 응용류식세포의분석성숙란포액중CD56~+NK세포급활화적CD56+NK세포적비례,결합IVF치료결과,탐토기상관성.결과 임신환자적란포액중평균CD56~+NK세포급활화적CD56~+NK세포분별위(15.57±3.10)%화(2.63±0.94)%,미임신환자적란포액중평균CD56~+NK세포급활화적CD56~+NK세포분별위(19.12±5.37)%화(4.06±2.08)%,현저고우임신조(P<0.05).결론 접수IVF조잉치료환자중성숙란포액중CD56~+NK세포급활화적CD56~+NK세포적비례교저자역획림상임신.
Objective To investigate the proportion of follicular fluid CD56~+ natural killer (NK) cells to the total lymphocytes and the ac-tivated CD56~+ NK cells to the total CD56~+ NK cells. To provide an evidence for improving the clinical pregnancy rate in in vitro fertilization (IVF) treatment by regulating the function of NK cells. Methods Triple color flow cytometry was used to analyze the proportion of CD56~+ NK cells and activated CD56~+ NK cells in mature follicular fluid. The IVF treatment outcome was closely followed up. The relationship be-tween the proportion of CD56~+ NK cells and activated CD56~+ NK cells and the IVF treatment outcome was analyzed statistically. Results The proportion of CD56~+ NK cells and activated CD56* NK cells in mature follicular fluid of women who got pregnancy by IVF treatment was (15.57±3.10)% and (2.63±0.94)% respectively,while the proportion of the women who didn't get pregnancy was (19.12±5.37)% and (4.06±2.08)% respectively,which was significantly higher than the pregnant group(P< 0.05). Conclusion The women with lower propor-tion of CD56~+ NK cells and activated CD56~+ NK cells in mature follicular fluid of is easier to get pregnancy by IVF. An altered NK cells pro-file in follicular fluid could therefore influence fertility in IVF treatment outcome.