四川大学学报（医学版）
四川大學學報（醫學版）
사천대학학보（의학판）
JOURNAL OF SICHUAN UNIVERSITY(MEDICAL SCIENCE EDITION)
2010年
2期
265-268
,共4页
TGF-β1%胚胎种植率%IFN-γ%IL-10
TGF-β1%胚胎種植率%IFN-γ%IL-10
TGF-β1%배태충식솔%IFN-γ%IL-10
TGF-β1%Implantation rate%IFN-γ%IL-10
目的 探讨TGF-β1在体外对小鼠胚胎发育及胚胎种植的影响及其作用机制.方法 ①收集小鼠2-细胞期胚胎,在含不同剂量TGF-β1的M16培养液中进行体外培养,观察胚胎发育情况,并进行胚胎移植,观察着床率;②测定移植后小鼠外周血及胚胎种植部位母胎界面IL-10/INF-γ水平.结果 ①1 ng/mL和10 ng/mL TGF-β1能提高2-细胞期胚胎体外发育速率、质量和胚胎着床率.其中TGF-β1 10 ng/mL时作用最显著,TGF-β1浓度过高对体外胚胎发育不利;②M16+TGF-β1 10 ng/mL组体外培养囊胚移植后母胎界面INF-γ低于M16组,差异有统计学意义[(30.89±11.31) pg/mL vs. (43.23±18.09) pg/mL,P<0.05].两组间IL-10水平无明显差异.结论 ①小鼠胚胎体外培养基中加入适宜浓度TGF-β1可提高2-细胞期胚胎体外发育速率、质量和胚胎着床率;②TGF-β1可能通过促进体外胚胎发育速率和质量,改善胚胎和内膜发育同步性,促进母胎界面Th1/Th2平衡向Th2偏移而促进胚胎着床.
目的 探討TGF-β1在體外對小鼠胚胎髮育及胚胎種植的影響及其作用機製.方法 ①收集小鼠2-細胞期胚胎,在含不同劑量TGF-β1的M16培養液中進行體外培養,觀察胚胎髮育情況,併進行胚胎移植,觀察著床率;②測定移植後小鼠外週血及胚胎種植部位母胎界麵IL-10/INF-γ水平.結果 ①1 ng/mL和10 ng/mL TGF-β1能提高2-細胞期胚胎體外髮育速率、質量和胚胎著床率.其中TGF-β1 10 ng/mL時作用最顯著,TGF-β1濃度過高對體外胚胎髮育不利;②M16+TGF-β1 10 ng/mL組體外培養囊胚移植後母胎界麵INF-γ低于M16組,差異有統計學意義[(30.89±11.31) pg/mL vs. (43.23±18.09) pg/mL,P<0.05].兩組間IL-10水平無明顯差異.結論 ①小鼠胚胎體外培養基中加入適宜濃度TGF-β1可提高2-細胞期胚胎體外髮育速率、質量和胚胎著床率;②TGF-β1可能通過促進體外胚胎髮育速率和質量,改善胚胎和內膜髮育同步性,促進母胎界麵Th1/Th2平衡嚮Th2偏移而促進胚胎著床.
목적 탐토TGF-β1재체외대소서배태발육급배태충식적영향급기작용궤제.방법 ①수집소서2-세포기배태,재함불동제량TGF-β1적M16배양액중진행체외배양,관찰배태발육정황,병진행배태이식,관찰착상솔;②측정이식후소서외주혈급배태충식부위모태계면IL-10/INF-γ수평.결과 ①1 ng/mL화10 ng/mL TGF-β1능제고2-세포기배태체외발육속솔、질량화배태착상솔.기중TGF-β1 10 ng/mL시작용최현저,TGF-β1농도과고대체외배태발육불리;②M16+TGF-β1 10 ng/mL조체외배양낭배이식후모태계면INF-γ저우M16조,차이유통계학의의[(30.89±11.31) pg/mL vs. (43.23±18.09) pg/mL,P<0.05].량조간IL-10수평무명현차이.결론 ①소서배태체외배양기중가입괄의농도TGF-β1가제고2-세포기배태체외발육속솔、질량화배태착상솔;②TGF-β1가능통과촉진체외배태발육속솔화질량,개선배태화내막발육동보성,촉진모태계면Th1/Th2평형향Th2편이이촉진배태착상.
Objective To investigate the role of TGF-β1 in embryo implantation and development in vitro in mice.Methods Mouse embryos at 2-cell stage were cultured in the media of M16 with exposure to different levels of TGF-β1 (0, 1, 10 and 50 ng/mL).The percentage of embryos reaching fixed stages (early blastocyst, expanding blastocyst and hatched blastocyst) was monitored 68 h and 92 h after the culture.The expanding blastocys cultured for 68 h in M16 without TGF-β1 and those with 10 ng/mL of TGF-β1 were transferred to pseudopregnant mice.On the 6~(th) day post transfer, the successful rates of implantation were counted.The level of IL-10/IFN-γ in the serum and maternal-fetus interface of the mice was detected by ELISA on the 6~(th) day post transfer.Results TGF-β1 improved embryo growth in vitro.TGF-β1 at a level of 10 ng/mL had the maximum impact, with 15.6%, 68.09%, 1.42% of embryos reaching early, expanding, and hatched stage, respectively, 68 h after culture, and 6.38%, 28.37%, 53.19% of embryos reaching early, expanding, and hatched stage, respectively, 92 h after culture.The promoting effect declined when TGF-β1 reached 50 ng/mL.The successful rate of implantation of embryos cultured in M16 with TGF-β1 was significantly higher than those cultured in M16 without TGF-β1 (35.42% vs.17.19%, P<0.05).The embryos cultured in M16 with TGF-β1 had significantly lower level of IFN-γ in the maternal-fetus interface than those cultured in M16 without TGF-β1 [(30.89±11.31) pg/mL vs.(43.23±18.09) pg/mL,P<0.05].Conclusion TGF-β1 at an appropriate dose improves embryo implantation in mice in vitro.The mechanism may involve the improvement of the quality of embryos and their development, and decrease of IFN-γ synthesis in maternal-fetal interface, a chemical that could cause Th2 bias.
