CAJ | 학술논문

为了解强筋优质小麦品种豫麦34的低分子量谷蛋白亚基(LMW-GS)的基因构成,利用普通小麦LMW-GS基因特异引物, 采用PCR扩增技术从中克隆得到一个LMW-GS新基因LMWY34 (GenBank No.GU183486).该基因具有LMW-GS基因的典型结构特征,编码区全长906 bp,编码302个氨基酸.推导氨基酸序列显示,LMWY34的编码产物N-端具有LMW-m 型低分子量谷蛋白亚基的典型特征,与已报道的GluD3-4位点编码的LMW-GS基因序列有很高的一致性(98.68%).
위료해강근우질소맥품충예맥34적저분자량곡단백아기(LMW-GS)적기인구성,이용보통소맥LMW-GS기인특이인물, 채용PCR확증기술종중극륭득도일개LMW-GS신기인LMWY34 (GenBank No.GU183486).해기인구유LMW-GS기인적전형결구특정,편마구전장906 bp,편마302개안기산.추도안기산서렬현시,LMWY34적편마산물N-단구유LMW-m 형저분자량곡단백아기적전형특정,여이보도적GluD3-4위점편마적LMW-GS기인서렬유흔고적일치성(98.68%).
In the present paper,a novel low-molecular-weight glutenin subunit (LMW-GS)gene, LMWY34 (GenBank No. GU183486),was amplified and cloned with a pair of specific primers of LMW-GS genes from the wheat variety Yumai 34. LMWY34 was a typical LMW-GS gene with whole coding region of 906 bp, which encoding a protein of 302 amino acid residues. Comparisons of amino acid sequences showed that it had the typical structure of LMW-m type in N-terminal region. It had high sequence similarity with the reported LMW-GS genes encoded by the GluD3-4 locus.