CAJ | 학술논문

目的建立高效液相色谱紫外检测法测定人血中瑞芬太尼(镇痛药)血药浓度.方法用1-氯丁J烷提取全血中的瑞芬太尼,选用GI97559(瑞芬太尼乙基类似物)为内标定量.色谱柱为Zobar SE-CN(4.6 mm × 250 mm,5 μm),流动相为乙腈-甲醇-磷酸二氢钾缓冲液,柱温30℃,流量1.5 mL·min~(-1),紫外榆测波长210 nm,按内标法定量.结果标准曲线在1～20 ng·mL~(-1) 范围内,浓度与峰面积比有良好的线性关系(γ=0.956).结论本方法操作简便、试剂成本低、灵敏度高、精密度好,为临床研究瑞芬太尼血药浓度提供一种较好的分析技术.
목적 건립고효액상색보자외검측법측정인혈중서분태니(진통약)혈약농도.방법 용1-록정J완제취전혈중적서분태니,선용GI97559(서분태니을기유사물)위내표정량.색보주위Zobar SE-CN(4.6 mm × 250 mm,5 μm),류동상위을정-갑순-린산이경갑완충액,주온30℃,류량1.5 mL·min~(-1),자외유측파장210 nm,안내표법정량.결과 표준곡선재1～20 ng·mL~(-1) 범위내,농도여봉면적비유량호적선성관계(γ=0.956).결론 본방법조작간편、시제성본저、령민도고、정밀도호,위림상연구서분태니혈약농도제공일충교호적분석기술.
Objective To establish an HPLC with UV method for determination of remifentanil in human blood.Methods Human blood with internal standard was extracted by butyl chloride.The sample was separated on Zobar SE-CN (4.6 mm × 250 mm,5μm) column with a mobile phase of mixture of acetonitrile,methanol and phosphate buffer,the temperature was set at 30℃ with flow rate of 1.5 mL·min~(-1).UV detection at 210 nm. Results The calibration curves were found to be linear at the range 1-20 ng·mL~(-1) of remifentanil in whole blood with a good correlation (γ=0.956).Conclusion This method is rapid,accurate and sensitive,which is very suitable for investigation of clinical blood concentration of remifentanil.