中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2010年
2期
109-112
,共4页
SD大鼠%骨质疏松动物模型%脑源性骨质疏松%低转换型骨质疏松
SD大鼠%骨質疏鬆動物模型%腦源性骨質疏鬆%低轉換型骨質疏鬆
SD대서%골질소송동물모형%뇌원성골질소송%저전환형골질소송
Sprague-Dawley (SD) rat%Osteoporosis model%Osteoporosis of brain origin%Low bone turnover osteoporosis
目的 研究大鼠脑源性骨质疏松的特性.方法 用雌性SD大鼠建立脑源性骨质疏松动物模型,用体内双荧光标记法测其右胫骨上段骨组织形态计量学动态参数,用XLA27153型流式细胞仪测定CD_3、CD_4、CD_8和NK细胞的百分率(%).用~3H-TdR掺入法测定大鼠体外T淋巴细胞转化功能.用放射免疫法测定血清中生长素(GH)、雌二醇(E_2)和骨钙素(BGP)的含量.用酶法测定血脂,称体脂质量.结果 与对照组比较,模型组大鼠:(1)代表骨形成的骨组织形态计量学动态参数值显著降低:荧光标记周长百分率(%L.PM),骨矿化沉积率(MAR),骨形成率(BFR/BS,BFR/BV)分别降低了33.6%(P＜0.01),25.5%(P＜0.01),48.0%(P＜0.01)和46.3%(P＜0.01);(2)生长素(GH)、雌二醇(E_2)和骨钙素(BGP)的含量显著降低;(3)免疫功能下降:CD_3、CD_4、CD_8、CD_4/CD_8、NK细胞百分率明显降低和体外T淋巴细胞转化能力明显降低;(4)脂质代谢紊乱:TC,TG,LDL-C显著升高,HDL-C显著降低,体脂质量显著增加.结论 大鼠脑源性骨质疏松是一种多因素的综合的神经、内分泌、免疫功能和脂质代谢功能减退和紊乱的低转换型骨质疏松.
目的 研究大鼠腦源性骨質疏鬆的特性.方法 用雌性SD大鼠建立腦源性骨質疏鬆動物模型,用體內雙熒光標記法測其右脛骨上段骨組織形態計量學動態參數,用XLA27153型流式細胞儀測定CD_3、CD_4、CD_8和NK細胞的百分率(%).用~3H-TdR摻入法測定大鼠體外T淋巴細胞轉化功能.用放射免疫法測定血清中生長素(GH)、雌二醇(E_2)和骨鈣素(BGP)的含量.用酶法測定血脂,稱體脂質量.結果 與對照組比較,模型組大鼠:(1)代錶骨形成的骨組織形態計量學動態參數值顯著降低:熒光標記週長百分率(%L.PM),骨礦化沉積率(MAR),骨形成率(BFR/BS,BFR/BV)分彆降低瞭33.6%(P＜0.01),25.5%(P＜0.01),48.0%(P＜0.01)和46.3%(P＜0.01);(2)生長素(GH)、雌二醇(E_2)和骨鈣素(BGP)的含量顯著降低;(3)免疫功能下降:CD_3、CD_4、CD_8、CD_4/CD_8、NK細胞百分率明顯降低和體外T淋巴細胞轉化能力明顯降低;(4)脂質代謝紊亂:TC,TG,LDL-C顯著升高,HDL-C顯著降低,體脂質量顯著增加.結論 大鼠腦源性骨質疏鬆是一種多因素的綜閤的神經、內分泌、免疫功能和脂質代謝功能減退和紊亂的低轉換型骨質疏鬆.
목적 연구대서뇌원성골질소송적특성.방법 용자성SD대서건립뇌원성골질소송동물모형,용체내쌍형광표기법측기우경골상단골조직형태계량학동태삼수,용XLA27153형류식세포의측정CD_3、CD_4、CD_8화NK세포적백분솔(%).용~3H-TdR참입법측정대서체외T림파세포전화공능.용방사면역법측정혈청중생장소(GH)、자이순(E_2)화골개소(BGP)적함량.용매법측정혈지,칭체지질량.결과 여대조조비교,모형조대서:(1)대표골형성적골조직형태계량학동태삼수치현저강저:형광표기주장백분솔(%L.PM),골광화침적솔(MAR),골형성솔(BFR/BS,BFR/BV)분별강저료33.6%(P＜0.01),25.5%(P＜0.01),48.0%(P＜0.01)화46.3%(P＜0.01);(2)생장소(GH)、자이순(E_2)화골개소(BGP)적함량현저강저;(3)면역공능하강:CD_3、CD_4、CD_8、CD_4/CD_8、NK세포백분솔명현강저화체외T림파세포전화능력명현강저;(4)지질대사문란:TC,TG,LDL-C현저승고,HDL-C현저강저,체지질량현저증가.결론 대서뇌원성골질소송시일충다인소적종합적신경、내분비、면역공능화지질대사공능감퇴화문란적저전환형골질소송.
Objective To investigate the characteristics of the osteoporosis of brain origin in rats.Methods (1) The model of osteoporosis of Brain Origin in female rats was established.(2) Morphological methods were used to estimate the bone histomorphometry parameters (3)Radioimmunoassay (RIA) was used to measure the level of the serum growth hormone (GH),estradiol (E_2) and bone Ga protein (BGP).(4) CD_3 (%),CD_4 (%),CD_8(%),CD_4/CD_8 (%) and NKC (%) were measured in rat peripheral blood.(5) ~3H-TdR was used to measure the changes of convertibility of T lymphocytes in rat peripheral blood.(6) Serum lipids and body fat weight were measured.Results Compared with control group,the model group rats:(1) Bone formation parameters decreased:% L.PM,MAR,BFR/BS,BFR/BV were decreased by33.6%(P＜0.01),25.5%(P＜0.01),48.0%(P＜0.01) and 46.3% (P ＜ 0.01) respectively;(2) The levels of serum E_2,GH,BGP significantly decreased;(3) CD_3,(%),CD_4 (%),CD_8 (%),CD_4/CD_8 (%) and NKC (%) significantly decreased,and convertibility of T lymphocytes decreased obviously;(4) Levels of total cholesterol (TC),triglyceride (TG),and low density lipoprotein-cholesterol (LDL-C) significantly increased,but concentration of high density lipoprotein-cholesterol (HDL-C) decreased obviously,and body fat weight significantly increased.Conclusion The osteoporosis of brain origin is the one of low bone turnover osteoporosis with the decrease of GH,E_2,BGP and immune function,and lipid metabolism disorder as well.
