CAJ | 학술논문

采用加热回流提取法、超声波辅助提取法、微波辅助萃取法和超临界CO_2流体提取等技术从厚朴枝中提取厚朴酚及和厚朴酚,优化提取条件,用反相高效液相色谱法(RP-HPLC)测定厚朴酚及和厚朴酚的提取得率,比较不同提取方法对厚朴枝中厚朴酚及和厚朴酚的提取效果.结果表明,微波辅助提取厚朴枝中厚朴酚速度最快,当微波功率为低火(1～2.8PV,200W)、乙醇体积分数65%、料液比1:40(mg/mL)、提取时间10min时,单次提取得率最高,厚朴酚与和厚朴酚及总酚得率分别为7.893mg/g和1.938mg/g.
채용가열회류제취법、초성파보조제취법、미파보조췌취법화초림계CO_2류체제취등기술종후박지중제취후박분급화후박분,우화제취조건,용반상고효액상색보법(RP-HPLC)측정후박분급화후박분적제취득솔,비교불동제취방법대후박지중후박분급화후박분적제취효과.결과표명,미파보조제취후박지중후박분속도최쾌,당미파공솔위저화(1～2.8PV,200W)、을순체적분수65%、료액비1:40(mg/mL)、제취시간10min시,단차제취득솔최고,후박분여화후박분급총분득솔분별위7.893mg/g화1.938mg/g.
Heat reflux,ultrasonic-assisted extraction,microwave-assisted extraction and supercritical carbon dioxide extraction were compared for extraction of magnolol and honokiol from branches of Megnolia Hypoleuca.Reverse-phase HPLC was used for quantitative analysis of magnolol and honokiol.Results showed that among four extraction techniques,the least time was consumed for the microwave-assisted extraction of magnolol and honokiol,and the single microwave-assisted extraction for 40 min at 200 W (1-2.8 PV) with 65% ethanol as extraction solvent using a material/liquid ratio of 1:40 (mg/mL) gave the highest yields of magnolol and honokiol,which were valued at 7.893 mg/g and 1.938 mg/g,respectively.