中国医学装备
中國醫學裝備
중국의학장비
CHINA MEDICAL EQUIPMENT
2011年
10期
35-37
,共3页
血液涂片%固定角度%标准化%改进
血液塗片%固定角度%標準化%改進
혈액도편%고정각도%표준화%개진
Blood smear slide%Fixed angle%Standardization%Improvement
目的：改进传统制作血液涂片技术,使其统一标准化,有效控制血液检验的质量。方法：制作10°～30°不同固定角度推片,用一定量的EDTA抗凝血制作血液涂片,观察血膜长度及染色后显微镜下细胞分布均匀度,并进行测量比较,找出符合制备标准角度的推片,制备标准血液涂片,与传统的制片法制作的血液涂片进行比对分析。结果：17°或18°固定角度推片,用血量5uL制作血液涂片,血膜长度X和S为4.081,3.987;0.015,0.0103;细胞分布均匀区X,S为3.033,2.971;0.015,0.0104。17°固定角度推片和传统的制备方法比较P〈0.001,有显著性差异。结论：17°或18°固定角度推片,用血量5uL制作的血液涂片符合标准血液涂片,在细胞形态检查中能有效进行质量控制,加大了对疾病诊断的准确率。
目的：改進傳統製作血液塗片技術,使其統一標準化,有效控製血液檢驗的質量。方法：製作10°～30°不同固定角度推片,用一定量的EDTA抗凝血製作血液塗片,觀察血膜長度及染色後顯微鏡下細胞分佈均勻度,併進行測量比較,找齣符閤製備標準角度的推片,製備標準血液塗片,與傳統的製片法製作的血液塗片進行比對分析。結果：17°或18°固定角度推片,用血量5uL製作血液塗片,血膜長度X和S為4.081,3.987;0.015,0.0103;細胞分佈均勻區X,S為3.033,2.971;0.015,0.0104。17°固定角度推片和傳統的製備方法比較P〈0.001,有顯著性差異。結論：17°或18°固定角度推片,用血量5uL製作的血液塗片符閤標準血液塗片,在細胞形態檢查中能有效進行質量控製,加大瞭對疾病診斷的準確率。
목적：개진전통제작혈액도편기술,사기통일표준화,유효공제혈액검험적질량。방법：제작10°～30°불동고정각도추편,용일정량적EDTA항응혈제작혈액도편,관찰혈막장도급염색후현미경하세포분포균균도,병진행측량비교,조출부합제비표준각도적추편,제비표준혈액도편,여전통적제편법제작적혈액도편진행비대분석。결과：17°혹18°고정각도추편,용혈량5uL제작혈액도편,혈막장도X화S위4.081,3.987;0.015,0.0103;세포분포균균구X,S위3.033,2.971;0.015,0.0104。17°고정각도추편화전통적제비방법비교P〈0.001,유현저성차이。결론：17°혹18°고정각도추편,용혈량5uL제작적혈액도편부합표준혈액도편,재세포형태검사중능유효진행질량공제,가대료대질병진단적준학솔。
Objective：To improve the traditional method of making blood smear slides to standardize the new method to effectively control the quality of blood tests.Methods：Using push-slides at an fixed angle of different degrees from 10° to 30° to make blood smear slides with a certain amount of EDTA anticoagulated blood.Under a microscope,the length of the blood membranes and the evenness of the distribution of cells were observed after staining the smear slides.Then measurement and comparison were performed to find out those smear slides which conform to established standards and identify the standard angles of push-slides.The standard blood smear slides were made using push-slides at the standard angles.The standard blood smear slides were compared with those made by the traditional method.Results：The blood smear slides were made with 5uL of blood using push-slides at an fixed angle of 17°/18° degree.The X and S values of membrane lengths for the slides were 4.081/3.987 and 0.015/0.0103,respectively.The X and S values for the areas of evenly distributed cells for the slides were 3.033/2.971 and 0.015/0.0104,respectively.The P value for the comparison between the slides by the traditional method and those by using push-slides at a fixed angle of 17° was less than 0.001,which means there is significant difference between these 2 kinds of slides.Conclusion：Blood smear slides made by using push-slides at a fixed angle of 17° or 18° with 5uL of blood satisfies the requirements for standard blood smear slides,and are therefore helpful in controlling the quality of morphological examination of blood cells,thus increasing the accuracy of diagnosis.
