中华现代护理杂志
中華現代護理雜誌
중화현대호리잡지
CHINESE JOURNAL OF MODERN NURSING
2011年
8期
909-911
,共3页
骨髓间充质干细胞%肌萎缩侧索硬化症%护理
骨髓間充質榦細胞%肌萎縮側索硬化癥%護理
골수간충질간세포%기위축측색경화증%호리
Bone mesenchymal stem cells%Amyotrophie lateral sclerosis%Nursing
目的 观察骨髓间充质干细胞(MSC)治疗肌萎缩侧索硬化症(ALS)的临床效果,探讨护理方法.方法 在无菌条件下采集患者骨髓液,密度梯度离心法分离,获取单个核细胞层,接种于培养瓶中,加入培养液,在5%二氧化碳细胞培养箱培养3d,微生物检测安全后静脉输注给患者.结果 治疗后能延迟肌萎缩侧索硬化症患者的病情进展,有效8例,无效1例.结论 MSC治疗肌萎缩侧索硬化症的临床效果有效.
目的 觀察骨髓間充質榦細胞(MSC)治療肌萎縮側索硬化癥(ALS)的臨床效果,探討護理方法.方法 在無菌條件下採集患者骨髓液,密度梯度離心法分離,穫取單箇覈細胞層,接種于培養瓶中,加入培養液,在5%二氧化碳細胞培養箱培養3d,微生物檢測安全後靜脈輸註給患者.結果 治療後能延遲肌萎縮側索硬化癥患者的病情進展,有效8例,無效1例.結論 MSC治療肌萎縮側索硬化癥的臨床效果有效.
목적 관찰골수간충질간세포(MSC)치료기위축측색경화증(ALS)적림상효과,탐토호리방법.방법 재무균조건하채집환자골수액,밀도제도리심법분리,획취단개핵세포층,접충우배양병중,가입배양액,재5%이양화탄세포배양상배양3d,미생물검측안전후정맥수주급환자.결과 치료후능연지기위축측색경화증환자적병정진전,유효8례,무효1례.결론 MSC치료기위축측색경화증적림상효과유효.
Objective To observe the therapeutic effects of bone marrow-derived mesenehymal stem cells(BMSCs)in the treatment of amyotrophie lateral sclerosis(ALS).Methods To collect bone marrow in asepsis conditions.Mononuclear cells were collected by gradient centrifugation,cultured in growth medium and 5%carbon dioxide for 3 days.BMSCs were analyzed by flow cytometry with a FACScan(Becton Dickinson). All BMSCs were transplanted into patients intra venous.Results After BMSCs treatment.8 patients with ALS had obvious clinical effects,1 patients had no response to treatment.Conclusions BMSCs treatment for ALS has obvious clinical effect.
