中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2012年
5期
462-466
,共5页
徐艳红%吴艺捷%罗越%徐先明
徐豔紅%吳藝捷%囉越%徐先明
서염홍%오예첩%라월%서선명
妊娠%甲状腺疾病%碘化甲状腺原氨酸脱碘酶%胎盘
妊娠%甲狀腺疾病%碘化甲狀腺原氨痠脫碘酶%胎盤
임신%갑상선질병%전화갑상선원안산탈전매%태반
Pregnant women%Thyroid disease%Iodothyronine deiodinase%Placenta
目的 探讨孕妇在不同甲状腺疾病及自身免疫状态时,胎盘Ⅱ型和Ⅲ型碘化甲状腺原氨酸脱碘酶(D2、D3)表达的变化.方法 选择处于不同甲状腺功能及自身免疫状态的孕妇为研究对象,并以既往无甲状腺疾病及相关疾病史、妊娠期甲状腺功能正常且抗甲状腺过氧化物酶抗体( TPOAb)阴性的孕妇作为对照,用RT-PCR法对所获得的足月产孕妇的胎盘组织进行D2、D3的检测,观察表达的变化.结果 胎盘组织中同时存在D2和D3的表达.在甲减未控制组产妇胎盘中,D2的相对灰度值为0.916±0.035,高于正常对照组的0.833±0.029(P＜ 0.05)；而D3的相对灰度值低于正常对照组(0.766±0.038与0.848±0.052),但差异无统计学意义(P＞0.05).妊娠前或妊娠中有甲亢或甲减的孕妇,经过有效治疗,分娩前甲状腺功能已控制在正常范围的产妇,其胎盘D2及D3的表达与正常对照组比较差异无统计学意义(P均＞0.05).甲状腺功能正常但TPOAb阳性的产妇,其胎盘D2和D3的相对灰度值分别为0.842±0.032、0.837±0.053,与正常对照组相比差异均无统计学意义(P均＞0.05).结论 人类胎盘组织中D2和D3并存.在母体甲减时,胎盘中D2的表达增强,D3的表达有所减弱,这种变化可能对稳定母体与胎儿间甲状腺激素输送起有益的作用.
目的 探討孕婦在不同甲狀腺疾病及自身免疫狀態時,胎盤Ⅱ型和Ⅲ型碘化甲狀腺原氨痠脫碘酶(D2、D3)錶達的變化.方法 選擇處于不同甲狀腺功能及自身免疫狀態的孕婦為研究對象,併以既往無甲狀腺疾病及相關疾病史、妊娠期甲狀腺功能正常且抗甲狀腺過氧化物酶抗體( TPOAb)陰性的孕婦作為對照,用RT-PCR法對所穫得的足月產孕婦的胎盤組織進行D2、D3的檢測,觀察錶達的變化.結果 胎盤組織中同時存在D2和D3的錶達.在甲減未控製組產婦胎盤中,D2的相對灰度值為0.916±0.035,高于正常對照組的0.833±0.029(P＜ 0.05)；而D3的相對灰度值低于正常對照組(0.766±0.038與0.848±0.052),但差異無統計學意義(P＞0.05).妊娠前或妊娠中有甲亢或甲減的孕婦,經過有效治療,分娩前甲狀腺功能已控製在正常範圍的產婦,其胎盤D2及D3的錶達與正常對照組比較差異無統計學意義(P均＞0.05).甲狀腺功能正常但TPOAb暘性的產婦,其胎盤D2和D3的相對灰度值分彆為0.842±0.032、0.837±0.053,與正常對照組相比差異均無統計學意義(P均＞0.05).結論 人類胎盤組織中D2和D3併存.在母體甲減時,胎盤中D2的錶達增彊,D3的錶達有所減弱,這種變化可能對穩定母體與胎兒間甲狀腺激素輸送起有益的作用.
목적 탐토잉부재불동갑상선질병급자신면역상태시,태반Ⅱ형화Ⅲ형전화갑상선원안산탈전매(D2、D3)표체적변화.방법 선택처우불동갑상선공능급자신면역상태적잉부위연구대상,병이기왕무갑상선질병급상관질병사、임신기갑상선공능정상차항갑상선과양화물매항체( TPOAb)음성적잉부작위대조,용RT-PCR법대소획득적족월산잉부적태반조직진행D2、D3적검측,관찰표체적변화.결과 태반조직중동시존재D2화D3적표체.재갑감미공제조산부태반중,D2적상대회도치위0.916±0.035,고우정상대조조적0.833±0.029(P＜ 0.05)；이D3적상대회도치저우정상대조조(0.766±0.038여0.848±0.052),단차이무통계학의의(P＞0.05).임신전혹임신중유갑항혹갑감적잉부,경과유효치료,분면전갑상선공능이공제재정상범위적산부,기태반D2급D3적표체여정상대조조비교차이무통계학의의(P균＞0.05).갑상선공능정상단TPOAb양성적산부,기태반D2화D3적상대회도치분별위0.842±0.032、0.837±0.053,여정상대조조상비차이균무통계학의의(P균＞0.05).결론 인류태반조직중D2화D3병존.재모체갑감시,태반중D2적표체증강,D3적표체유소감약,저충변화가능대은정모체여태인간갑상선격소수송기유익적작용.
Objective To investigate the expression of type Ⅱ and type Ⅲ iodothyronine deiodinases (D2 and D3 ) of human placenta on pregnant women with different thyroid diseases and different autoimmunity.Methods Pregnant women with different thyroid diseases and autoimmunity were selected into the experiment group,and pregnant women who had no individual or family history of thyroid diseases with normal thyroid function and negative thyroid peroxidase antibody (TPOAb) were selected into the control group.Expression level of iodothyronine deiodinase( D2 and D3 )on placenta was measured by RT-PCR.Results D2 and D3 were both expressed on human placenta.D2 showed significantly higher expression level in hypothyroidism uncontrolled group than that in control group ( 0.916 ± 0.035 vs 0.833 ± 0.029,P ＜ 0.05 ),however,D3 showed a lower expression(0.766 ±0.038 vs 0.848 ±0.052),on placenta and the difference was not significant(P ＞ 0.05 ).In those who had history of hyperthyroidism or hypothyroidism but whose thyroid function become normal by effective treatment,D2 and D3 expression exhibited no difference from that of the control(P ＞ 0.05).In those pregnant women with normal thyroid function and TPOAb-positive,D2 and D3 expression was (0.842 ± 0.032 ) and ( 0.837 ± 0.053 ) respectively and there was also no difference from that of the control( P ＞ 0.05 ).Conclusion D2 and D3 were simultaneously expressed on human placenta.In those pregnant women with hypothyroidism,the level of D2 expression is high and the level of D3 expression is low.Those changes might be important and helpful for the stabilization of thyroid hormone transportation between mother and fetus.
