中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2009年
10期
905-908
,共4页
氯氮平%子代大鼠%生长发育%旷场行为%海马%MAPK44/42%Pho-MAPK44/42
氯氮平%子代大鼠%生長髮育%曠場行為%海馬%MAPK44/42%Pho-MAPK44/42
록담평%자대대서%생장발육%광장행위%해마%MAPK44/42%Pho-MAPK44/42
Clozapine%Filial rats%Development%Open field%Hippocampus%MAPK44/42%Pho-MAPK4-4/42
目的 建立孕期氯氮平暴露子代大鼠模型,观察孕期给药对子代造成的影响.方法 8周龄wistar雌鼠,分为对照组(n=17只)和用药组(n=25只),在大鼠孕6～15d,对照组腹腔注射生理盐水,用药组注射氯氮平针剂.观察子代大鼠体格发育、神经反射、神经行为以及脑MAPK表达.结果 氯氮平孕期暴露组导敛子代大鼠早期生长延迟,出生后第4天和第7天(F=12.56/7.51,P＜0.01)体质量增长低于对照组;神经发育指标显示悬崖回避反射在出生后第3天和第9天(F=4.969/4.348;P＜0.05)与对照组相比回避反应时间延长;空中翻正反射达标数目较对照组明显减少(F=7.959/6.457,P＜0.01/0.05);旷场行为试验未显示差异性;对出生后15d龄的子代大鼠海马及其前额叶皮质MAPK44/42表达检测显示:海马部位Pho-MAPK44/42表达显著低于对照组(F=18.729/23.824,P＜0.01),MAPK44/42表达2组差异无显著性;前额叶皮质Pho-MAPK44/42和MAPK44/42表达2组差异无显著性.结论 孕期氯氮平暴露可导致子代大鼠生长发育和脑发育异常.
目的 建立孕期氯氮平暴露子代大鼠模型,觀察孕期給藥對子代造成的影響.方法 8週齡wistar雌鼠,分為對照組(n=17隻)和用藥組(n=25隻),在大鼠孕6～15d,對照組腹腔註射生理鹽水,用藥組註射氯氮平針劑.觀察子代大鼠體格髮育、神經反射、神經行為以及腦MAPK錶達.結果 氯氮平孕期暴露組導斂子代大鼠早期生長延遲,齣生後第4天和第7天(F=12.56/7.51,P＜0.01)體質量增長低于對照組;神經髮育指標顯示懸崖迴避反射在齣生後第3天和第9天(F=4.969/4.348;P＜0.05)與對照組相比迴避反應時間延長;空中翻正反射達標數目較對照組明顯減少(F=7.959/6.457,P＜0.01/0.05);曠場行為試驗未顯示差異性;對齣生後15d齡的子代大鼠海馬及其前額葉皮質MAPK44/42錶達檢測顯示:海馬部位Pho-MAPK44/42錶達顯著低于對照組(F=18.729/23.824,P＜0.01),MAPK44/42錶達2組差異無顯著性;前額葉皮質Pho-MAPK44/42和MAPK44/42錶達2組差異無顯著性.結論 孕期氯氮平暴露可導緻子代大鼠生長髮育和腦髮育異常.
목적 건립잉기록담평폭로자대대서모형,관찰잉기급약대자대조성적영향.방법 8주령wistar자서,분위대조조(n=17지)화용약조(n=25지),재대서잉6～15d,대조조복강주사생리염수,용약조주사록담평침제.관찰자대대서체격발육、신경반사、신경행위이급뇌MAPK표체.결과 록담평잉기폭로조도렴자대대서조기생장연지,출생후제4천화제7천(F=12.56/7.51,P＜0.01)체질량증장저우대조조;신경발육지표현시현애회피반사재출생후제3천화제9천(F=4.969/4.348;P＜0.05)여대조조상비회피반응시간연장;공중번정반사체표수목교대조조명현감소(F=7.959/6.457,P＜0.01/0.05);광장행위시험미현시차이성;대출생후15d령적자대대서해마급기전액협피질MAPK44/42표체검측현시:해마부위Pho-MAPK44/42표체현저저우대조조(F=18.729/23.824,P＜0.01),MAPK44/42표체2조차이무현저성;전액협피질Pho-MAPK44/42화MAPK44/42표체2조차이무현저성.결론 잉기록담평폭로가도치자대대서생장발육화뇌발육이상.
Objective To set up the experimental animal model of the filial rats exposed clozapine in the pregnant clay and expect to know what is the effect on the offspring exposed the antipsychiatrie in gestation.Methods Female Wister rats were divided into both the control group (n=17)and elozapine group (n=2.5) which were intraperitoneally injected saline and clozapine respectively from pregnant day 6 to day 15,and their pup rats were examined development indexes such as the physical growth ,neural reflex,neural behavior and the expression of MAPK44/42 in brain in neonatal stage.Results it was shown that clozapine- exposed in pregnant day lead to the retardation of the weight of filial rats in earlier age such as postnatal day 4 (PND4) and PND7(F=12.56/7.51,P=0.001/0.008).As for neural reflex,the time of cliff avoidance of rats was significantly delayed in PND 3 and day 9 (F=4.969/4.348 ; P=0.03/0.041),and the ratio of positive response of air righting decreased compared with the control group in PND13 and PND15 (F=7.959/6.475,P=0.007/0.016).There was no difference between two groups on the behavior of the filial rats in the open field of filial rats.The expression of PhoMAPK44/42 in hippocampi of exposed-clozapine offspring in PND15 was significantly lower than the control' s(F=18.729/23.824,P=0.001) ,however,no difference was demonstrated on the expression of MAPK44/42 in hippocamaias well as the expression of both Pho-MAPK44/42 and MAPK44/42 in forehead cortex in two groups.Conclusion The resuits suggest the aberration of both physical and mental development of filial rats exposed clozapine in gestation.
