CAJ | 학술논문

目的探讨兔脊髓损伤模型损伤后双后肢肌力和脊髓运动诱发电位D波之间的关系.方法 45只兔按随机数字表法分为打击组(显露硬脊膜并打击,并按打击能量大小分为50gcf组、75 gcf组、100gcf组、125 gcf组、150gcf组、175 gcf组、200 gcf组、250 gcf组)和对照组(显露硬脊膜但不打击).打击后和第4周末记录兔双后肢肌力、运动诱发电位D波的潜伏期和波幅,并在第4周末取家兔脊髓行神经微丝(NF)免疫组化染色及病理形态观察,测量NF吸光度值.结果脊髓损伤当时和第4周末脊髓硬膜外运动诱发电位D波波幅下降的幅度与兔后肢运动功能呈线性相关关系(R2=0.98,P＜0.001,回归曲线Y=0.33+4.82X;R2=0.99,P＜0.001,回归曲线Y=0.04+0.51X),而D波潜伏期的变化则与术后肌力的变化无相关关系(R2=-0097,P=0.573;R2=-0.165,P=0.337);NF吸光度值随着D波波幅的下降而下降,两者呈线性相关关系(R2=0.87,P＜0.001,回归曲线Y=0.12+0.58X).结论在脊髓损伤中可以通过脊髓硬膜外运动诱发电位D波波幅的下降程度作为判断脊髓损伤运动功能障碍程度的指标.
목적 탐토토척수손상모형손상후쌍후지기력화척수운동유발전위D파지간적관계.방법 45지토안수궤수자표법분위타격조(현로경척막병타격,병안타격능량대소분위50gcf조、75 gcf조、100gcf조、125 gcf조、150gcf조、175 gcf조、200 gcf조、250 gcf조)화대조조(현로경척막단불타격).타격후화제4주말기록토쌍후지기력、운동유발전위D파적잠복기화파폭,병재제4주말취가토척수행신경미사(NF)면역조화염색급병리형태관찰,측량NF흡광도치.결과 척수손상당시화제4주말척수경막외운동유발전위D파파폭하강적폭도여토후지운동공능정선성상관관계(R2=0.98,P＜0.001,회귀곡선Y=0.33+4.82X;R2=0.99,P＜0.001,회귀곡선Y=0.04+0.51X),이D파잠복기적변화칙여술후기력적변화무상관관계(R2=-0097,P=0.573;R2=-0.165,P=0.337);NF흡광도치수착D파파폭적하강이하강,량자정선성상관관계(R2=0.87,P＜0.001,회귀곡선Y=0.12+0.58X).결론 재척수손상중가이통과척수경막외운동유발전위D파파폭적하강정도작위판단척수손상운동공능장애정도적지표.
Objective To study the relationship between D-wave of thc spinal motor evoked potential and hindlimb motor function status (myodynamia) of rabbits with experimental spinal cord injury. Methods Forty-five rabbits were randomly divided into a control group and 8 injured groups (receiving Allen's injury of50, 75, 100, 125, 150, 175, 200 and 250 gcf, respectively). The myodynamia of hindlimbs and the latency and amplitude of D-wave of the spinal motor evoked potential were recorded right after the injury and in the 4th week of injury, respectively. Simultaneously in the 4th week of injury,the rabbits' spinal sections were stained with neurofilament (NF) immunohistochemistry and their pathological morphologies were observed; and optical densities of NF were measured. Statistic analysis of correlation between myodynamia of hindlimbs and latency and amplitude of D-wave was performed.Results A statistic linear correlation was found between amplitudes declination of D-wave of the spinal motor evoked potential and modified Tarlov's scores right after the injury and in the 4th week of injury (R2=0.98, P＜0.00, regression curve: Y=0.33+4.82X; R=0.99, P＜0.001, regression curve: Y=0.04+0.5 1X),but no statistical correlation was noted between myodynamia and the latency of D-wave of the spinal motor evoked potential at the same time periods(R2=-0.097, P=0.573; R2=-0.165, P=0.337). And the optical density of NF decreased following the amplitude declination of D-wave of the spinal motor evoked potential with linear correlation (R2=0.87, P＜0.001, regression curve: Y= 0.12+0.58X).Conclusion Amplitude declination of D-wave of the spinal motor evoked potential could be used to evaluate the severity of dyskinesia in the injured spinal cord.