CAJ | 학술논문

目的观察重组人内皮抑素对结肠癌细胞SW620增殖和凋亡的影响.方法在培养液中加入梯度浓度的重组人内皮抑素(0.1、0.5、1.0,5.0、10.0、20.0mg/L)分别作用24、48、72 h,采用噻唑蓝(MTT)比色法检测细胞生长,流式细胞术检测细胞周期和凋亡,扫描及透射电镜观察细胞超微结构的变化.结果内皮抑素对SW620细胞的增殖有抑制作用.其中,作用48 h的抑制率分别为13.46%、15.38%、20.82%、24.71%、30.12%和37.44%,呈剂量依赖关系.给药后24 h和48 h,内皮抑素组细胞G0/G1期比例增高,而s期细胞比例降低(P＜0.05).72h两组差异无统计学意义(P＞0.05).24 h和48 h的凋亡率分别为(1.430±0.145)%和(1.760±0.054)%,均高于对照组(0.670±0.181)%和(1.260±0.138)%(P＜0.05).透射电子显微镜下,内皮抑素组细胞微绒毛减少,染色质厚薄不均,位于核膜下.结论重组内皮抑素可以在体外直接抑制结肠癌细胞的增殖,诱导凋亡,影响细胞骨架等结构.
목적 관찰중조인내피억소대결장암세포SW620증식화조망적영향.방법 재배양액중가입제도농도적중조인내피억소(0.1、0.5、1.0,5.0、10.0、20.0mg/L)분별작용24、48、72 h,채용새서람(MTT)비색법검측세포생장,류식세포술검측세포주기화조망,소묘급투사전경관찰세포초미결구적변화.결과 내피억소대SW620세포적증식유억제작용.기중,작용48 h적억제솔분별위13.46%、15.38%、20.82%、24.71%、30.12%화37.44%,정제량의뢰관계.급약후24 h화48 h,내피억소조세포G0/G1기비례증고,이s기세포비례강저(P＜0.05).72h량조차이무통계학의의(P＞0.05).24 h화48 h적조망솔분별위(1.430±0.145)%화(1.760±0.054)%,균고우대조조(0.670±0.181)%화(1.260±0.138)%(P＜0.05).투사전자현미경하,내피억소조세포미융모감소,염색질후박불균,위우핵막하.결론 중조내피억소가이재체외직접억제결장암세포적증식,유도조망,영향세포골가등결구.
Objective To investigate the effect of endostatin on the proliferation of colon cancer SW620 cells. Methods The SW620 cells were treated by recombinant human endostatin(0. l ,0. 5,1.0,5.0,10.0,20.0 mg/L). The effect of endostatin on the proliferation, cell cycle, apoptosis and ultrastructure of SW620 cells were examined after 24,48 and 72 h. Results The proliferation of SW620 cells were inhibited by endostatin compared with the control group. The inhibiting rates were 13. 46% ,15. 38% ,20. 82% ,24. 71% ,30. 12% and 37.44% .respectively (P ＜0.05). Endostatin arrested SW620 cells at G0/G1 phase (P ＜ 0. 05); The apoptotic rates were (1.430 ± 0. 145) % and (1. 760 ± 0. 054) % in endostatin group after 24 and 48 h, respectively. This was a significant increase when compared with the control group after 24 and 48 h (0. 670 ±0. 181)% and (1.260 ±0. 138)% (P＜0. 05). Under TEM,both the number of protrusions and microfilaments of SW620 cells in the endostatin group decreased. Conclusion Recombination human endostatin inhibits the proliferation of SW620 cells,induce the apoptosis,and changes the skeleton of SW620 cells directly.