中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2008年
9期
636-639
,共4页
于霄云%钟媛%牛玉红%曲春清%李革新%吕秀强%孙贵范%金亚平
于霄雲%鐘媛%牛玉紅%麯春清%李革新%呂秀彊%孫貴範%金亞平
우소운%종원%우옥홍%곡춘청%리혁신%려수강%손귀범%금아평
谷胱甘肽%亚硒酸钠%砷%甲基化
穀胱甘肽%亞硒痠鈉%砷%甲基化
곡광감태%아서산납%신%갑기화
Glutathione%Sodium selenite%Arsenic%Methylation
目的 探讨给予外源性谷胱甘肽(GSH)和亚硒酸钠(sodium selenite)对饮水砷暴露小鼠肝、肾和血中砷代谢的影响.方法 将小鼠按数字表法随机分为对照组、单纯染砷组(砷组)、GSH干预组(GSH组)与亚硒酸钠干预组(硒组),每组各8只小鼠.小鼠以自由饮水方式共染砷4周,饮水砷浓度为50 mg/L.从第4周起,染砷同时腹腔注射GSH(600 mg/kg体重)或亚硒酸钠(1 mg/kg体重)进行干预,共干预7 d.末次注射后处死小鼠,取其肝、肾和血组织样品.采用氢化物发生-超低温捕集-原子吸收分光光度法,分别检测小鼠肝、肾和血中无机砷(iAs)、一甲基胂(MMA)和二甲基胂(DMA)的含量.结果 GSH组小鼠肝中DMA含量[(233.76±60.63)ng/g湿重]及血中DMA含量[(88.52±30.86)ng/g湿重]和总砷(TAs)含量[(162.32±49.45)ng/g湿重]高于相对应的砷组小鼠[(218.36±42.71)、(45.32±12.19)、(108.51±18.00)ng/g湿重](q值分别为3.06、6.40、10.72,P<0.05).GSH组小鼠肝中砷一甲基化率(PMI,0.65±0.05)和二甲基化率(SMI,0.55±0.05)及血中PMI(0.85±0.07)与砷组小鼠相对应的甲基化率(0.58±0.06、0.44±0.09、0.54±0.11)比较升高(q值分别为3.75、5.26、4.21.P<0.05).硒组与砷组各项指标间差异无统计学意义.结论 给予外源性GSH可以促进iAs在小鼠体内甲基化代谢,从而降低其对机体的毒性损伤.而亚硒酸钠则无明显作用.
目的 探討給予外源性穀胱甘肽(GSH)和亞硒痠鈉(sodium selenite)對飲水砷暴露小鼠肝、腎和血中砷代謝的影響.方法 將小鼠按數字錶法隨機分為對照組、單純染砷組(砷組)、GSH榦預組(GSH組)與亞硒痠鈉榦預組(硒組),每組各8隻小鼠.小鼠以自由飲水方式共染砷4週,飲水砷濃度為50 mg/L.從第4週起,染砷同時腹腔註射GSH(600 mg/kg體重)或亞硒痠鈉(1 mg/kg體重)進行榦預,共榦預7 d.末次註射後處死小鼠,取其肝、腎和血組織樣品.採用氫化物髮生-超低溫捕集-原子吸收分光光度法,分彆檢測小鼠肝、腎和血中無機砷(iAs)、一甲基胂(MMA)和二甲基胂(DMA)的含量.結果 GSH組小鼠肝中DMA含量[(233.76±60.63)ng/g濕重]及血中DMA含量[(88.52±30.86)ng/g濕重]和總砷(TAs)含量[(162.32±49.45)ng/g濕重]高于相對應的砷組小鼠[(218.36±42.71)、(45.32±12.19)、(108.51±18.00)ng/g濕重](q值分彆為3.06、6.40、10.72,P<0.05).GSH組小鼠肝中砷一甲基化率(PMI,0.65±0.05)和二甲基化率(SMI,0.55±0.05)及血中PMI(0.85±0.07)與砷組小鼠相對應的甲基化率(0.58±0.06、0.44±0.09、0.54±0.11)比較升高(q值分彆為3.75、5.26、4.21.P<0.05).硒組與砷組各項指標間差異無統計學意義.結論 給予外源性GSH可以促進iAs在小鼠體內甲基化代謝,從而降低其對機體的毒性損傷.而亞硒痠鈉則無明顯作用.
목적 탐토급여외원성곡광감태(GSH)화아서산납(sodium selenite)대음수신폭로소서간、신화혈중신대사적영향.방법 장소서안수자표법수궤분위대조조、단순염신조(신조)、GSH간예조(GSH조)여아서산납간예조(서조),매조각8지소서.소서이자유음수방식공염신4주,음수신농도위50 mg/L.종제4주기,염신동시복강주사GSH(600 mg/kg체중)혹아서산납(1 mg/kg체중)진행간예,공간예7 d.말차주사후처사소서,취기간、신화혈조직양품.채용경화물발생-초저온포집-원자흡수분광광도법,분별검측소서간、신화혈중무궤신(iAs)、일갑기신(MMA)화이갑기신(DMA)적함량.결과 GSH조소서간중DMA함량[(233.76±60.63)ng/g습중]급혈중DMA함량[(88.52±30.86)ng/g습중]화총신(TAs)함량[(162.32±49.45)ng/g습중]고우상대응적신조소서[(218.36±42.71)、(45.32±12.19)、(108.51±18.00)ng/g습중](q치분별위3.06、6.40、10.72,P<0.05).GSH조소서간중신일갑기화솔(PMI,0.65±0.05)화이갑기화솔(SMI,0.55±0.05)급혈중PMI(0.85±0.07)여신조소서상대응적갑기화솔(0.58±0.06、0.44±0.09、0.54±0.11)비교승고(q치분별위3.75、5.26、4.21.P<0.05).서조여신조각항지표간차이무통계학의의.결론 급여외원성GSH가이촉진iAs재소서체내갑기화대사,종이강저기대궤체적독성손상.이아서산납칙무명현작용.
Objective To explore the effect of glutathione(GSH)and sodium selenite on the metabolism of arsenic in the liver,kidney and blood of mice exposed to iAsⅢ through drinking water.Methods The mice were randomly divided into control,arsenic,GSH and sodium selenite group,respectively.And each group had eight mice and the mice were exposed to 50 mg/L arsenite by drinking water for 4 weeks.Mice were intraperitoneally injected with GSH(600 mg/kg)and sodium selenite (1 mg/kg)for seven days from the beginning of the fourth week.At the end of the fourth week,liver,kidney and blood were samplod to assess the concentrations of inorganic arsenic(iAs),monomethylarsenic acid(MMA),dimethylarsenic acid(DMA)by hydride generation trapping by ultra-hypothermia coupled with atomic absorption spectrometry.Results The liver DMA(233.76±60.63 ng/g)concentration in GSH group was significantly higher than the arsenic group(218.36±42.71 ng/g).The concentration of DMA (88.52±30.86 ng/g)and total arenic(Tas)(162.32±49.45 ng/g)in blood of GSH group was significantly higher than those[(45.32±12.19 ng/g),(108.51±18.00 ng/g),respectively]of arsenic groups(q values were 3.06,6.40,10.72 respectively,P<0.05).The primary methylated index(PMI)(0.65±0.050)and secondary methyhted index(SMI)(0.55±0.050)in liver sample of GSH group were significantly higher than those(0.58±0.056,0.44±0.093)in arsenic group.In blood samples,the PMI (0.85±0.066)in GSH group was significantly higher than that(0.54±0.113)in arsenic group(q values were 3.75,5.26,4.21 respectively,P<0.05).However,no significant difference was identified between sodium selenite and arsenic groups in liver,kidney or blood samples.And no significant difference was detected in kidney samples among all arsenic exposing groups.Conclusion Exogenous GSH could promote the methylated metabolism of iAsⅢ,but sodium selenite showed no significant effects.