CAJ | 학술논문

目的观察生长激素(GH)作用前后胰腺癌细胞中GH-磷酸化Janus激酶2(pJAK2)信号转导通路的特征,探讨GH对胰腺癌细胞作用的分子机制.方法不同浓度的GH(50、100 μg/L)作用于胰腺癌细胞(SW-1990、Cap-1、ASPC),计数培养24、48、72h后的细胞数;收集SW-1990注射于裸鼠背部皮下,成瘤后随机分为注射GH的实验组(GH组,共21只,每日4 mg/kg共2周,1、2、24 h取材,每组7只)及注入盐水的对照组(NS组,7只),注射期间隔日测量瘤体积.Western blot方法观察GH作用后不同时间(体外,GH 50μg/L:5、10、15、30、45min、1、2 h;体内:1、2、24 h)胰腺癌细胞pJAK2表达变化.结果 GH可刺激SW-1990、Cap-1、ASPC增殖但在体内不促进肿瘤牛长:pJAK2在7株胰腺细胞(SW-1990、Cap-1、Colo、Mia、Aspc、P3、Panc-1)均有不同程度的表达;与细胞毒T淋巴细胞(CTL)比较,GH刺激后pJAK2表达显著增强(5、10、15 min尤为明显),但1、2 h有减弱趋势;GH应用于裸鼠后各时间点(1、2、24 h)pJAK2在移植瘤中的表达差异无统计学意义.结论 GH能促进胰腺癌细胞SW-1990、Cap-1、ASPC增殖,但在体内不刺激肿瘤生长;GH可显著增强pJAK2在SW-1990中的表达,而在体内这种变化不明显.
목적 관찰생장격소(GH)작용전후이선암세포중GH-린산화Janus격매2(pJAK2)신호전도통로적특정,탐토GH대이선암세포작용적분자궤제.방법 불동농도적GH(50、100 μg/L)작용우이선암세포(SW-1990、Cap-1、ASPC),계수배양24、48、72h후적세포수;수집SW-1990주사우라서배부피하,성류후수궤분위주사GH적실험조(GH조,공21지,매일4 mg/kg공2주,1、2、24 h취재,매조7지)급주입염수적대조조(NS조,7지),주사기간격일측량류체적.Western blot방법관찰GH작용후불동시간(체외,GH 50μg/L:5、10、15、30、45min、1、2 h;체내:1、2、24 h)이선암세포pJAK2표체변화.결과 GH가자격SW-1990、Cap-1、ASPC증식단재체내불촉진종류우장:pJAK2재7주이선세포(SW-1990、Cap-1、Colo、Mia、Aspc、P3、Panc-1)균유불동정도적표체;여세포독T림파세포(CTL)비교,GH자격후pJAK2표체현저증강(5、10、15 min우위명현),단1、2 h유감약추세;GH응용우라서후각시간점(1、2、24 h)pJAK2재이식류중적표체차이무통계학의의.결론 GH능촉진이선암세포SW-1990、Cap-1、ASPC증식,단재체내불자격종류생장;GH가현저증강pJAK2재SW-1990중적표체,이재체내저충변화불명현.
Objective To observe the impact of growth hormone (GH) on expression of phosphoJAK2 (pJAK2) in pancreatic carcinoma cells and to discuss significance of pJAK2 in signaling.Methods (1)Pancreatic carcinoma cells (SW-1990,Cap-1,ASPC) during exponential growth stage were harvested and cultured in medium containing growth hormone(50 μg/L,100μg/L);After 24,48,72 hours,cells were counted using a Coulter Counter.(2)Athymic nude BALB/C mice were inoculated with SW-1990 cells,When tumors became palpable after inoculation.animals were randomized to receive GH(4 mg/kg.s.c.once daily for 2 weeks,n=21)versus saline control cytotoxic lymphocyte(CTL,n=7).(3)Human pancreatic carcinoma cell lines(SW-1990,Cap-1,Colo,Mia,Aspc,P3,Panc-1) were collected to detect the basal expression of pJAK2 with immol/lunoblots;After GH administration on special times(in vitro:5,10,15,30,45 min,1,2 h;in vivo:1,2,24 h),Cells were collected and Tumor tissues of the mice were rapidly incised respectively.All the specimen were frozen immol/lediately for subsequent Western blotting analysis,to ohserve the impact of GH on expression of pJAK2 in SW-1990 and inoculation tumor cells.Results The results revealed GH stimulated cell growth in vitro;On immol/lunoblots,positive expression of pJAK2 was observed on human pancreatic carcinoma cell lines(SW-1990、Cap-1、Colo、Mia、Aspc、P3、Panc-1).GH augmented rapidly tyrosine phosphorylation of this special proteins to the highest value on the 5th minute after GH administration in vitro and the values fluctuated later;No similar phenomena,however,were observed in vivo.Conclusion Positive expression of pJAK2 is observed on human pancreatic cancer ceils;Furthermore,GH augments rapidly tyrosine phosphorylation of these special proteins not in vivo but in vitro;and this discrepancy helps to explain why GH doesn't accelerate tumor growth in vivo.