CAJ | 학술논문

目的观察纤维连接蛋白(FN)、层粘连蛋白(LN)和胶原蛋白Ⅳ(COL Ⅳ)在大鼠膀胱癌组织的表达.方法选取正常SD大鼠膀胱组织及大鼠原位膀胱癌模型建立后的非肌层浸润性膀胱癌(NMIBC)组织标本(每组20例),用免疫荧光及测定单位面积荧光强度(F/S)的方法比较FN、LN和COLⅣ表达的变化.结果 FN和LN在NMIBC的表达与正常大鼠膀胱组织比较,F/S值分别为FN(6.91 ±1.39和0.82±0.22)、LN(6.57±2.39和0.90±0.46),差异有统计学意义(P＜0.05)；COLⅣ在NMIBC和正常大鼠膀胱组织F/S值为1.05±0.57和0.84±0.37,差异无统计学意义(P＞0.05).结论 FN和LN在NMIBC表达明显升高,COL Ⅳ的表达差异无统计学意义.
목적 관찰섬유련접단백(FN)、층점련단백(LN)화효원단백Ⅳ(COL Ⅳ)재대서방광암조직적표체.방법 선취정상SD대서방광조직급대서원위방광암모형건립후적비기층침윤성방광암(NMIBC)조직표본(매조20례),용면역형광급측정단위면적형광강도(F/S)적방법비교FN、LN화COLⅣ표체적변화.결과 FN화LN재NMIBC적표체여정상대서방광조직비교,F/S치분별위FN(6.91 ±1.39화0.82±0.22)、LN(6.57±2.39화0.90±0.46),차이유통계학의의(P＜0.05)；COLⅣ재NMIBC화정상대서방광조직F/S치위1.05±0.57화0.84±0.37,차이무통계학의의(P＞0.05).결론 FN화LN재NMIBC표체명현승고,COL Ⅳ적표체차이무통계학의의.
Objective To study the expression change of extracellular matrix in rat bladder cancer.Methods The bladder specimens of normal SD rats ( control group) and the non-muscle invasive bladder cancer (NMIBC) specimens in the established orthotopic rat model of bladder cancer (test group) (20specimens per group) were chosen.By using immunofluorescence and fluorescence intensity per unit area (F/S),the expression change of fibronectin ( FN ),laminin (LN) and collagen Ⅳ was investigated.Results As compared with control group,the fluorescent staining of fibronectin and laminin in non-muscle invasive bladder cancer was obviously increased,and the F/S values had statistically significant difference between test group and control group ( PFN ＜ 0.01 and PLN ＜ 0.01 ) with FN ( 6.91 ± 1.39 and 0.82 ±0.22),LN (6.57 ± 2.39 and 0.90 ± 0.46),respectively.The fluorescent staining of collagen Ⅳ and the F/S value had no significant difference between test group and control group ( P ＞ 0.05 ).Conclusion The expression of fibronectin and laminin in non-muscle invasive bladder cancer was obviously increased,nd there was no significant difference in the expression of COL Ⅳ between non-muscle invasive bladder cancer and rat bladder normal tissue.