CAJ | 학술논문

目的观察重组变构人肿瘤坏死因子相关凋亡诱导配体(rmhTRAIL)与吉两他滨(GEM)联合应用对人非小细胞肺癌细胞株NCI-H460在体内外的抑制作用.方法采用二苯基溴化四氮唑蓝(MTT)法,检测系列浓度的rmhTRAIL和GEM单独或联合应用时对人肺癌NCI-H460细胞生长的抑制作用.将NCI-H460细胞裸鼠移植瘤模型分为6组:对照组(腹腔注射生理盐水)、rmhTRAIL组、GEM组、rmhTRAIL+GEM组、GEM+顺铂(DDP)组和rmhTRAIL+GEM+DDP组,分别给药.每3～4 d测量1次裸鼠移植瘤大小,用药后21 d,处死裸鼠,剥取肿瘤称重.结果 rmhTRAIL和GEM单独或联合应用时对NCI-H460细胞的生长抑制作用呈剂量依赖性,且系列浓度的rmhTRAIL和GEM的联合具有协同抑制作用.rmhTRAIL组、rmhTRAIL+GEM组、GEM+DDP组和rmhTRAIL+GEM+DDP组的相对肿瘤体积分别为4.75±3.04、2.53±1.25、4.52±2.87和1.69±0.97,均显著低于对照组(8.82±5.62,均P＜0.05);肿瘤重量分别为(2.23±0.29)g、(1.12±0.77)g、(2.51±0.87)g和(0.60±0.18)g,均显著低于对照组[(4.71±0.97)g,均P＜0.01].rmhTRAIL+GEM组的相对肿瘤体积和肿瘤苇量均显著低于rmhTRAIL组和GEM组(P＜0.05和P＜0.01).rmhTRAIL+GEM+DDP组的相对肿瘤体积和肿瘤重量均显著低于rmhTRAIL组和GEM+DDP组(P＜0.05和P＜0.01).结论在体内外实验中,rmhTRAIL与吉西他滨联合对人肺癌细胞株NCI-H460的生长均显示出协同抑制作用.
목적 관찰중조변구인종류배사인자상관조망유도배체(rmhTRAIL)여길량타빈(GEM)연합응용대인비소세포폐암세포주NCI-H460재체내외적억제작용.방법 채용이분기추화사담서람(MTT)법,검측계렬농도적rmhTRAIL화GEM단독혹연합응용시대인폐암NCI-H460세포생장적억제작용.장NCI-H460세포라서이식류모형분위6조:대조조(복강주사생리염수)、rmhTRAIL조、GEM조、rmhTRAIL+GEM조、GEM+순박(DDP)조화rmhTRAIL+GEM+DDP조,분별급약.매3～4 d측량1차라서이식류대소,용약후21 d,처사라서,박취종류칭중.결과 rmhTRAIL화GEM단독혹연합응용시대NCI-H460세포적생장억제작용정제량의뢰성,차계렬농도적rmhTRAIL화GEM적연합구유협동억제작용.rmhTRAIL조、rmhTRAIL+GEM조、GEM+DDP조화rmhTRAIL+GEM+DDP조적상대종류체적분별위4.75±3.04、2.53±1.25、4.52±2.87화1.69±0.97,균현저저우대조조(8.82±5.62,균P＜0.05);종류중량분별위(2.23±0.29)g、(1.12±0.77)g、(2.51±0.87)g화(0.60±0.18)g,균현저저우대조조[(4.71±0.97)g,균P＜0.01].rmhTRAIL+GEM조적상대종류체적화종류위량균현저저우rmhTRAIL조화GEM조(P＜0.05화P＜0.01).rmhTRAIL+GEM+DDP조적상대종류체적화종류중량균현저저우rmhTRAIL조화GEM+DDP조(P＜0.05화P＜0.01).결론 재체내외실험중,rmhTRAIL여길서타빈연합대인폐암세포주NCI-H460적생장균현시출협동억제작용.
Objective To evaluate the inhibitory effects of recombinant mutant tumor necrosis factor-related apoptosis.inducing ligand(rmhTRAIL)combined with chemotherapeutic agent gemeitabine (GEM)on human lung cancer cell line NCI-H460 cells in vitro and in vivo. Methods MTT was used to evaluate the cvtotoxic effects of rmhTRAIL and GEM either used alone or in combination treatment on NCIH460 cells.BAL B/c nude mice were transplanted with NCI-H460 tumor.The tumor-bearing nude mice were randomly divided into 6 groups(n=6):negative control group(to be injected intraperitoneally with normal sallne);rmhTRAIL group;GEM group;rmhTRAIL plus GEM group;GEM plus DDP group;rmhTRAIL plus GEM andDDP group.The tumor size was measured every 3-4 days.Twenty one days after the administration of different drugs the mice were killed and the tumors were taken out and weighed.Results The growth inhibition of NCI-H460 cells was dose.dependent after exposure to rmhTRAIL, GEM alone or together.The combination of mhTRAIL and GEM showed a synergistic inhibitory effect at different concentrations.The relative tumor volume of rmhTRAIL group,rmhTRAIL plus GEM group,GEM plus DDP group and nnhTRAIL plus GEM and DDP group were 4.75±3.04,2.53±1.25,4.52±2.87,and 1.69±0.97,respectively.all significantly smaller than that of the negative control group(8.82±5.62,P＜0.05 or P＜0.01).The tumor weight of these four groups were(2.23±0.29)g,(1.12±0.77)g,(2.51±0.87) g,and 0.60±0.18 g,respectively,all significantly less then that of the negative control group (4.71 g±0.97 g,all P ＜0.01 ).Both the relative tumor volume and tumor weight of rmhTRAIL plus GEM group were significantly smaller than those of either rmhTRAIL group or GEM group (P＜0.05 and P＜0.01,respectively).Both the relative tumor volume and tumor weight of rmhTRAIL plus GEM and DDP group were significantly smaller than those of either rmhTRAIL group or GEM plus DDP group (P＜0.05 and P＜0.01,respectively).Conclusion The combination of rmhTRAIL and GEM has a synergistic inhibitory effect on human NSCLC cell line NCI-H460 cells either in vitro and in vivo.