CAJ | 학술논문

目的研究冻存大鼠胚胎神经干细胞(NSCs)异体移植对脊髓损伤(SCI)的修复作用.方法 45只Wistar大鼠于SCI后2 d随机分为A、B、C 3组,每组15只,分别在SCI处注入相同体积的生理盐水、NSCs、低温冻存后复苏的NSCs.术后分别通过免疫组化和组织切片鉴定移植细胞的分化与存活、BBB运动评分和斜板实验评定大鼠功能恢复,细胞存活率,以及评价NSCs低温冻存情况.结果免疫组化及组织切片显示,B、C组在移植区均有NSCs的特异性表达nestin阳性细胞存活,而A组无表达;脊髓空洞面积A组[(3.9±0.1)mm2]与B组[(1.2±0.3)mm2]和C组[(1.1±0.3)mm2]比较,组间差异有统计学意义(F=423.949,P<0.01),而B、C组间的差异无统计学意义(P＞0.05).BBB评分:A组[(2.0±0.5)分]与B组[(16.4±0.8)分]和C组[(16.0±1.4)分]间的差异有统计学意义(F=970.157,P<0.01),但B、C组间差异无统计学意义(P＞0.05);斜板实验评分:A组[(31.3±2.9)度]与B组[(46.8±2.1)度]和C组[(46.5±2.4)度]间的差异有统计学意义(F=151.099,P<0.01),B、C组间差异无统计学意义(P＞0.05).低温冻存后复苏液的NSCs细胞存活率[(55.9±5.2)%]与未冻存[(65.1±3.4)%]差异无统计学意义(t=3.334,P＞0.05).结论冻存NSCs复苏后仍具有活跃的增殖和分化潜能,是SCI移植治疗有价值的细胞资源.
목적 연구동존대서배태신경간세포(NSCs)이체이식대척수손상(SCI)적수복작용.방법 45지Wistar대서우SCI후2 d수궤분위A、B、C 3조,매조15지,분별재SCI처주입상동체적적생리염수、NSCs、저온동존후복소적NSCs.술후분별통과면역조화화조직절편감정이식세포적분화여존활、BBB운동평분화사판실험평정대서공능회복,세포존활솔,이급평개NSCs저온동존정황.결과 면역조화급조직절편현시,B、C조재이식구균유NSCs적특이성표체nestin양성세포존활,이A조무표체;척수공동면적A조[(3.9±0.1)mm2]여B조[(1.2±0.3)mm2]화C조[(1.1±0.3)mm2]비교,조간차이유통계학의의(F=423.949,P<0.01),이B、C조간적차이무통계학의의(P＞0.05).BBB평분:A조[(2.0±0.5)분]여B조[(16.4±0.8)분]화C조[(16.0±1.4)분]간적차이유통계학의의(F=970.157,P<0.01),단B、C조간차이무통계학의의(P＞0.05);사판실험평분:A조[(31.3±2.9)도]여B조[(46.8±2.1)도]화C조[(46.5±2.4)도]간적차이유통계학의의(F=151.099,P<0.01),B、C조간차이무통계학의의(P＞0.05).저온동존후복소액적NSCs세포존활솔[(55.9±5.2)%]여미동존[(65.1±3.4)%]차이무통계학의의(t=3.334,P＞0.05).결론동존NSCs복소후잉구유활약적증식화분화잠능,시SCI이식치료유개치적세포자원.
Objective To observe the therapeutic effect of spinal cord injury(SCI)by transplantation cryopreserved neural stem ceils(NSCs).Methods 45 Wistar rats were randomly divided into three groups.with 15 rats in each group.Transplantations were carried out two days after SCI,group A:0.9% saline water,group B:NSCs,group C:cryopreserved NSCs.After the transplantation,immunohistochemical and tissue incision were appeared to detect the survival and differentiation of the transplanted ceils in nivo,and the methods of BBB and oblique plate were used to estimate the recovery of function.Results Group B and C all had active NSCs except group A.The injuried spinal cord A[(3.9±0.1)mm2],B[(1.2±0.3)mm2]and C[(1.1±0.3)mm2],there was an variance in the three groups (F=423.949,P＜0.01),BBB test:group A[(2.0±0.5)mark],B[(16.4±0.8)mark]and C [(16.0±1.4)mark],there was an variance in the three groups(F=970.157,P＜0.01),the oblique plate test:group A[(31.3±2.9)degree],B[(46.8±2.1)degree]and C[(46.5.4-2.4)degree],there was an variance in the three groups(F=151.099,P＜0.01),the tests demonstrate there were no variance in group B and C(all P＞0.05),there was no variance in living rate between the cryopreserved NSCs group [(55.9±5.2)%]and no eryopreserved[(65.1±3.4)%,t=3.334,P＞0.05].Conclusions Cryopreserved NSCs keep the ability of reproducdon and differentiation.NSCs is a kind of valuable cell resource for the therapy of SCI.