CAJ | 학술논문

为探讨海马mu型阿片肽受体介导癫痫发作敏感性形成的作用,实验采用微渗透泵技术,观察大鼠腹侧海马注射mu型阿片肽受体激动剂PL017(2.09、2.59、3.29μg/μ1)、拮抗剂β-funaltrexamine hydrochloride(β-FNA、0.88、1.10、1.35μg/μl)对红藻氨酸(kainic acid,KA)诱导癫痫发作的干预作用.PL017能够明显缩短癫痫发作潜伏期、增加癫痫发作级别(P＜0.05),β-FNA则可显著延长癫痫发作潜伏期、降低发作级别(P＜0.01);PL017和β-FNA的干预作用均表现出剂量依赖效应.结果表明,海马mu型阿片肽受体具有促进KA诱导的癫痫发作敏感性形成作用.
위탐토해마mu형아편태수체개도전간발작민감성형성적작용,실험채용미삼투빙기술,관찰대서복측해마주사mu형아편태수체격동제PL017(2.09、2.59、3.29μg/μ1)、길항제β-funaltrexamine hydrochloride(β-FNA、0.88、1.10、1.35μg/μl)대홍조안산(kainic acid,KA)유도전간발작적간예작용.PL017능구명현축단전간발작잠복기、증가전간발작급별(P＜0.05),β-FNA칙가현저연장전간발작잠복기、강저발작급별(P＜0.01);PL017화β-FNA적간예작용균표현출제량의뢰효응.결과표명,해마mu형아편태수체구유촉진KA유도적전간발작민감성형성작용.
There is evidence that 5～7 d after acute seizure episodes induced by kainic acid (KA) the rats develop a long-lasting increase in the susceptibility to seizures followed by spontaneous recurrent seizures (SRS). The present study was focused on the role of hippocampal mu opioid receptors (MORs) in the susceptibility of rats to seizures with the KA model of epilepsy. The rats received a convulsant dose of KA (10 mg/kg, i.p.) were continuously infused with a selective MOR agonist PL017 (2.09, 2.59, 3.29μg/μl), or a selective MOR antagonist β-funaltrexamine hydrochloride (β-FNA, 0.88, 1.10, and 1.35 μg/μl) into ventral hippocampus by means of mini-osmotic pumps. Seven days later, the susceptibility of rats to seizures was checked by a subconvulsant dose of KA (5 mg/kg, i.p.). PL017 infusion shortened the latency and increased the stage of seizures induced by subconvulsant dose of KA in a dose-dependent manner. In contrast, infusion of β-FNA exhibited a dose-dependent effect against seizures challenged by subconvulsant dose of KA. These results indicate that hippocampal MOR may exert a promoting effect on the susceptibility of rats to KA-induced seizures.