中国神经科学杂志
中國神經科學雜誌
중국신경과학잡지
CHINESE JOURNAL OF NEUROSCIENCE
2004年
5期
331-335
,共5页
屈金良%刘涛%庞有旺%李金莲
屈金良%劉濤%龐有旺%李金蓮
굴금량%류도%방유왕%리금련
腰髓%背根神经节%坐骨神经%Ⅰ型囊泡膜谷氨酸转运体%免疫组织化学%大鼠
腰髓%揹根神經節%坐骨神經%Ⅰ型囊泡膜穀氨痠轉運體%免疫組織化學%大鼠
요수%배근신경절%좌골신경%Ⅰ형낭포막곡안산전운체%면역조직화학%대서
lumbar spinal cord%dorsal root ganglia%sciatic nerve%VGluT1%Immunohistochemistry%rat
目的研究单侧坐骨神经结扎对大鼠腰4～5脊髓节段和相应的背根神经节(DRGs)内VGluT1样免疫阳性反应产物表达的影响以及VGluT1通过轴浆流向外周转运的情况.方法采用免疫组织化学方法观察单侧坐骨神经结扎后不同时间内腰4～5脊髓节段、DRGs和结扎部位的近、远侧端神经干内VGLuT1样免疫阳性反应强度的变化.结果(1)坐骨神经结扎后第1和第2天,VGluT1样免疫阳性产物在结扎的同侧腰4～5脊髓节段和相应节段的DRGs内未检测到明显变化;但自术后第4天开始,可观察到VGluT1样免疫阳性产物的表达在上述部位逐渐减弱;VGluT1样免疫阳性产物表达的降低在上述部位所出现的时间和程度相平行.(2)结扎后第1天即可观察到VGluT1样免疫阳性产物在坐骨神经结扎部位近侧端的表达有所升高,但自术后第4天开始逐渐降低;而VGluT1样免疫阳性产物在坐骨神经结扎部位远侧端的表达自结扎后第1天起就逐渐降低,至第4周时已完全消失.结论(1)DRG神经元合成VGluT1,并通过轴浆流将VGLluT1向中枢突和周围突运输,故腰髓内部分VGluT1样阳性末梢起源于DRG神经元;(2)外周神经的损伤很易影响到DRG神经元内VGluT1的合成.
目的研究單側坐骨神經結扎對大鼠腰4～5脊髓節段和相應的揹根神經節(DRGs)內VGluT1樣免疫暘性反應產物錶達的影響以及VGluT1通過軸漿流嚮外週轉運的情況.方法採用免疫組織化學方法觀察單側坐骨神經結扎後不同時間內腰4～5脊髓節段、DRGs和結扎部位的近、遠側耑神經榦內VGLuT1樣免疫暘性反應彊度的變化.結果(1)坐骨神經結扎後第1和第2天,VGluT1樣免疫暘性產物在結扎的同側腰4～5脊髓節段和相應節段的DRGs內未檢測到明顯變化;但自術後第4天開始,可觀察到VGluT1樣免疫暘性產物的錶達在上述部位逐漸減弱;VGluT1樣免疫暘性產物錶達的降低在上述部位所齣現的時間和程度相平行.(2)結扎後第1天即可觀察到VGluT1樣免疫暘性產物在坐骨神經結扎部位近側耑的錶達有所升高,但自術後第4天開始逐漸降低;而VGluT1樣免疫暘性產物在坐骨神經結扎部位遠側耑的錶達自結扎後第1天起就逐漸降低,至第4週時已完全消失.結論(1)DRG神經元閤成VGluT1,併通過軸漿流將VGLluT1嚮中樞突和週圍突運輸,故腰髓內部分VGluT1樣暘性末梢起源于DRG神經元;(2)外週神經的損傷很易影響到DRG神經元內VGluT1的閤成.
목적연구단측좌골신경결찰대대서요4～5척수절단화상응적배근신경절(DRGs)내VGluT1양면역양성반응산물표체적영향이급VGluT1통과축장류향외주전운적정황.방법채용면역조직화학방법관찰단측좌골신경결찰후불동시간내요4～5척수절단、DRGs화결찰부위적근、원측단신경간내VGLuT1양면역양성반응강도적변화.결과(1)좌골신경결찰후제1화제2천,VGluT1양면역양성산물재결찰적동측요4～5척수절단화상응절단적DRGs내미검측도명현변화;단자술후제4천개시,가관찰도VGluT1양면역양성산물적표체재상술부위축점감약;VGluT1양면역양성산물표체적강저재상술부위소출현적시간화정도상평행.(2)결찰후제1천즉가관찰도VGluT1양면역양성산물재좌골신경결찰부위근측단적표체유소승고,단자술후제4천개시축점강저;이VGluT1양면역양성산물재좌골신경결찰부위원측단적표체자결찰후제1천기취축점강저,지제4주시이완전소실.결론(1)DRG신경원합성VGluT1,병통과축장류장VGLluT1향중추돌화주위돌운수,고요수내부분VGluT1양양성말소기원우DRG신경원;(2)외주신경적손상흔역영향도DRG신경원내VGluT1적합성.
Objective To study the effects of complete ligation of the unilateral sciatic nerve ( SN ) on the expression of VGluT1 -like immunoreactivity ( VGluT1 -LI ) in the fourth and fifth lumbar spinal cord ( L4, L5 ) , corresponding dorsal root ganglia (DRGs) and transport of VGluT1-LI by axonal flow peripherally through peripheral axons of DRG neurons.Method Immunohistochemistry was used to examine the change of VGluT1-LI in the L4 and L5, corresponding DRGs and the nerve stump proximal or distal to ligation in the different times after unilateral ligation of the SN. Results ( 1 )Obvious change of VGluT1-LI in the neuronal terminals of ipsilateral L4, L5 and cell bodies of DRGs was not detected in the first 2 d after complete ligation of the unilateral SN. But from the 4th day (4 d ) after ligation, a persistent decrease of VGluT1-LI was shown in the neuronal terminals ipsilateral L4, L5 and corresponding DRG neurons. The reduction of VGluT1 -LI in the spinal cord ( SC ) was paralleled with that in DRG neurons in the time and degree; ( 2 ) VGluT1-LI was observed to increase in the nerve stump proximal to ligation 1 d after unilateral ligation of the SN, but to decrease persistently in the nerve stump proximal to ligation from 4 d. On the other hand, VGluT1-LI decreased persistently in the nerve stump distal to ligation from 1 d and disappeared from 4 d after unilateral ligation of the SN. Conclusion ( 1 ) VGluT1was synthesized in cell bodies of DRG neurons and was transported centrally and peripherally by axonal flow. Therefore,part of the VGluT1 in the SC originated from DRG neurons; ( 2 ) VGluT1 synthesis in the DRGs was vulnearable to damage of the peripheral axons.
