中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2009年
7期
619-622
,共4页
梁红茹%刘晓慧%陈晶%孙招金%郭霄峰
樑紅茹%劉曉慧%陳晶%孫招金%郭霄峰
량홍여%류효혜%진정%손초금%곽소봉
狂犬病毒%绿色荧光蛋白%快速荧光斑点抑制实验
狂犬病毒%綠色熒光蛋白%快速熒光斑點抑製實驗
광견병독%록색형광단백%쾌속형광반점억제실험
rabies virus%green fluorescent protein%rapid fluorescent focus inhibition test
目的 建立一种新型的快速荧光斑点抑制实验用于狂犬病毒抗体的检测.方法 本研究以携带绿色荧光蛋白基因的嵌合狂犬病毒HEP-GFP株为基础毒株,建立了一种新型快速荧光斑点抑制实验(RFFIT-GFP);并对多份人、犬、猫的血清进行了检测,还将该检测结果与传统的RFFIT,ELISA相比较.结果 与结论 RFFIT-GFP和RFFIT,ELISA测定的结果基本相一致,特异性都比较高,而且RFFIT-GFP是一种比它们更为简便、经济的检测方法.
目的 建立一種新型的快速熒光斑點抑製實驗用于狂犬病毒抗體的檢測.方法 本研究以攜帶綠色熒光蛋白基因的嵌閤狂犬病毒HEP-GFP株為基礎毒株,建立瞭一種新型快速熒光斑點抑製實驗(RFFIT-GFP);併對多份人、犬、貓的血清進行瞭檢測,還將該檢測結果與傳統的RFFIT,ELISA相比較.結果 與結論 RFFIT-GFP和RFFIT,ELISA測定的結果基本相一緻,特異性都比較高,而且RFFIT-GFP是一種比它們更為簡便、經濟的檢測方法.
목적 건립일충신형적쾌속형광반점억제실험용우광견병독항체적검측.방법 본연구이휴대록색형광단백기인적감합광견병독HEP-GFP주위기출독주,건립료일충신형쾌속형광반점억제실험(RFFIT-GFP);병대다빈인、견、묘적혈청진행료검측,환장해검측결과여전통적RFFIT,ELISA상비교.결과 여결론 RFFIT-GFP화RFFIT,ELISA측정적결과기본상일치,특이성도비교고,이차RFFIT-GFP시일충비타문경위간편、경제적검측방법.
The objective of the present study was to establish a novel rapid fluorescence focus inhibition test (RFFIT GFP)for the detection of rabies virus antibody, in which a chimeric rabies virus expressing green fluorescent protein (HEP GFP) was used as the basic virus strain in RFFIT GFP assay, and a few serum samples from human, dog and cat were detected by this new method .The optimal serum dilution, virus dosage and infection time were determined in 24 serum samples from human, dog and cat by using RFFIT GFP, RFFIT and ELISA assays. The result showed that these 3 methods gave a good consistency. But RFFIT GFP was found to be more convenient and economic for the detection of rabies virus antibody.
