CAJ | 학술논문

目的:研究转录因子c-jun及下游蛋白MMP-9在胃癌组织中的表达状况,分析其与临床病理特征和生存之间的关系.方法:应用免疫组化SP法,采用组织芯片技术,分析189例胃癌组织、54例癌旁组织、41例淋巴结转移灶及32例正常对照组织中c-Jun和MMP-9蛋白表达.结果:胃癌中c-Jan和MMP-9蛋白的阳性表达率分别为73.0%和78.3%.c-Jun蛋白的表达与分化程度有关(P<0.05);MMP-9的表达与浸润深度、淋巴结转移、分化程度及Lauren分型有关(P<0.05).胃癌中c-Jun和MMP-9蛋白表达水平呈正相关(P<0.05).41例淋巴结转移灶和相对应的41例胃癌组织各因子表达比较发现,MMP-9阳性表达率有显著性差异(P<0.05),c-Jun阳性表达率无显著性差异(P>0.05).单因素生存分析显示c-Jun和MMP-9阳性组对胃癌患者生存期的影响有统计学意义(P<0.05),COX多元回归分析显示c-Jun和MMP-9的异常表达均不可作为影响胃癌预后的独立因素(P>0.05).结论:c-Jun和MMP-9在胃癌组织中均有高表达.c-Jun阳性表达与胃癌的组织学分级密切相关,提示其异常表达可能是胃癌发生过程中的一个早期分子事件.MMP-9阳性表达与肿瘤的浸润、转移密切相关,可作为判断胃癌恶性行为的重要生物学标记物.c-Jun和MMP-9蛋白表达水平呈正相关.c-Jan和MMP-9蛋白阳性表达对于胃癌患者是一个不利的预后因素,尚不能作为一个独立的预后指标.
목적:연구전록인자c-jun급하유단백MMP-9재위암조직중적표체상황,분석기여림상병리특정화생존지간적관계.방법:응용면역조화SP법,채용조직심편기술,분석189례위암조직、54례암방조직、41례림파결전이조급32례정상대조조직중c-Jun화MMP-9단백표체.결과:위암중c-Jan화MMP-9단백적양성표체솔분별위73.0%화78.3%.c-Jun단백적표체여분화정도유관(P<0.05);MMP-9적표체여침윤심도、림파결전이、분화정도급Lauren분형유관(P<0.05).위암중c-Jun화MMP-9단백표체수평정정상관(P<0.05).41례림파결전이조화상대응적41례위암조직각인자표체비교발현,MMP-9양성표체솔유현저성차이(P<0.05),c-Jun양성표체솔무현저성차이(P>0.05).단인소생존분석현시c-Jun화MMP-9양성조대위암환자생존기적영향유통계학의의(P<0.05),COX다원회귀분석현시c-Jun화MMP-9적이상표체균불가작위영향위암예후적독립인소(P>0.05).결론:c-Jun화MMP-9재위암조직중균유고표체.c-Jun양성표체여위암적조직학분급밀절상관,제시기이상표체가능시위암발생과정중적일개조기분자사건.MMP-9양성표체여종류적침윤、전이밀절상관,가작위판단위암악성행위적중요생물학표기물.c-Jun화MMP-9단백표체수평정정상관.c-Jan화MMP-9단백양성표체대우위암환자시일개불리적예후인소,상불능작위일개독립적예후지표.
Objective:To investigate the expression of c-Jun and MMP-9 in gastric cancer tissues,para-cancerous tissues and metastastic lymph nodes,and to explore its role and significance for the clinicopathology and prognosis.Methods:Immunohistochemistry was employed to detect the expression of c-Jun and MMP-9 in tissue microarrays containing gastric normal mucosa(n=32),para-cancerous tissues(n=54),metastastic lymph nodes(n=41),and gastric cancer tissues(n=189).Results:The positive rates for c-Jun and MMP-9 expression in gastric cancer were 73.0%and 78.3%,respectively.The positive rates of c-Jun protein was significantly associated with the degree of differentiation(P<0.05),but was not associated with the depth of invasion,lymph node metastasis,Lauren type,sex,age or size of tumor(P>0.05).The positive rates of MMP-9 was significantly associated with the depth of invasion,lymph node metastasis,Lauren type and degree of differentiation(P<0.05),but was not associated with sex,age or size of tumor(P>0.05).The positive rates of MMP-9 expression in the 41 gastnc cancer tissue samples and 41 metastastic lymph node tissue samples were significantly different(P<0.05).In metastastic lymph nodes,the positive rate of MMP-9 expression was higher.Kaplan-Meier survival analysis showed that the survival rate of patients with negative c-Jun and MMP-9 expression was higher than that of patients with positive c-Jun and MMP-9 expression(P<0.05).COX regression analysis showed that c-Jun and MMP-9 expressioh was not independent prognostic factor for gastric cancer. Conclusion:The expression of c-Jun is positively associated with the degree of differentiation.The increased c-Jun expression maybe an early indicator of gastric Cancer. The high expression of MMP-9 may involve the Occurrence,development,invasion,and metastasis of gastric cancer. C-Jun and MMP-9 are useful markers for predicting the outcome of gastric cancer,but they are not independent prognostic factors.