CAJ | 학술논문

采用对甲氧基苯磺酰氯作为柱前衍生试剂,RP-HPLC为分析模式,建立了一种新的生物胺衍生化方法,并对葡萄酒中生物胺含量进行检测. 通过液质联用对产物进行定性,研究并确定了最适衍生化条件:衍生温度50 ℃,缓冲液pH 9.0,衍生时间15 min. 实验建立了7种生物胺的HPLC分离方法:BeckmanODS柱;流动相A为10 mmol/L的NH_4Ac溶液(pH 6.37),B相为乙腈;采用梯度洗脱;流速1 mL/min;检测波长240 nm, 室温.
채용대갑양기분광선록작위주전연생시제,RP-HPLC위분석모식,건립료일충신적생물알연생화방법,병대포도주중생물알함량진행검측. 통과액질련용대산물진행정성,연구병학정료최괄연생화조건:연생온도50 ℃,완충액pH 9.0,연생시간15 min. 실험건립료7충생물알적HPLC분리방법:BeckmanODS주;류동상A위10 mmol/L적NH_4Ac용액(pH 6.37),B상위을정;채용제도세탈;류속1 mL/min;검측파장240 nm, 실온.
A novel derivative method for the determination of biogenic amines has been developed by use of 4-methoxy benzenesulphonyl chloride. The derivate production identification was performed by HPLC-MS system. The optimized conditions of derivatization were listed as following: the reaction temperature was 50 ℃;the pH value was 9 and the reaction time was 15 min. The chromatographic conditions were as following: Beckman ODS column (5 μm, 4.6×150 mm),the detection wavelength was 240 nm,the flow rate was 1.0 mL/min;Mobile phase A: 10 mmol/L NH_4Ac (pH 6.37), Mobile phase B: ACN;the gradient protocol was applied. The method has many advantages such as sensitivity, simplicity (pmol), rapidity and stability, which is suitable for the derivatization of biogenic amines and other substances which have amino group.