CAJ | 학술논문

目的:筛选出产碱性木聚糖酶酶活力高的菌株.方法:从碱性环境中采集样品,以自制木聚糖为惟一利碳源,采用刚果红透明圈法于摇瓶发酵法相结合筛选出18株产木聚糖酶酶活较高的菌株,其中1株酶活最高可达335.14 IU/ml.结果:通过培养特性及形态特征初步鉴定为芽孢杆菌.对部分酶学性质研究的结果表明:其作用最适温度60℃,最适pH8.0,在pH9.0条件下仍具有80%酶活力.结论:属于耐碱性木聚糖酶,具有很好的工业应用前景.
목적:사선출산감성목취당매매활력고적균주.방법:종감성배경중채집양품,이자제목취당위유일리탄원,채용강과홍투명권법우요병발효법상결합사선출18주산목취당매매활교고적균주,기중1주매활최고가체335.14 IU/ml.결과:통과배양특성급형태특정초보감정위아포간균.대부분매학성질연구적결과표명:기작용최괄온도60℃,최괄pH8.0,재pH9.0조건하잉구유80%매활력.결론:속우내감성목취당매,구유흔호적공업응용전경.
Objective: Screening produces alkaline xylanase enzyme activity of high strain. Method: 18 xylanase producing strains were obtained from alkaline condition by assaying congo - red hydrolyzed circle on the selective medium, and xylanase activity under shake -flask fermentation, in which xylan were used as the only carbon source, and the maximum enzyme activity achieved 335.14 IU/ml. It was tentatively identified as Bacillus based on its morphological characterization. Result: The study on characteristics of xylanase was carried out.The results show that the optimal reaction condition of xylanase were 60℃ and pH8.0, and its activity remained 80% of maximum activity at pH 9.0. Conclusion:It exhibit favorable potential for application in the processing industries.