CAJ | 학술논문

目的:研究中药丹参的主要成分丹参素对肝星状细胞(hepatic stellate cell,HSC)活化与增殖的影响.方法:乙醛与HSC-T6共同孵育诱导细胞活化,再分别应用不同浓度的丹参素进行干预.四甲基偶氮唑盐(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT)比色法检测细胞活力,流式细胞术检测细胞周期,实时定量聚合酶链反应法分析细胞Ⅰ型纤溶酶原激活剂抑制物( plasminogen activatorinhibitor-1,PAI-1)、转化生长因子β1(transforming growth factor-β1,TGF-β1)、尿激酶型纤溶酶原激活因子(urokinase-type plasminogen activator,uPA)和基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)的基因表达.结果:MTT比色法检测表明丹参素可抑制由200 μmol/L乙醛诱导的HSC增殖,流式细胞术分析发现丹参素可使处于S期的细胞数量明显增加(P＜0.05).此外,丹参素还能下调TGF-β1和PAI-1的基因表达,并增加uPA的转录水平(P＜0.01),但对MMP-2的表达没有明显影响.结论:丹参素可抑制乙醛诱导的HSC-T6的活化与增殖,并可调节参与细胞外基质沉积的细胞因子的表达.
목적:연구중약단삼적주요성분단삼소대간성상세포(hepatic stellate cell,HSC)활화여증식적영향.방법:을철여HSC-T6공동부육유도세포활화,재분별응용불동농도적단삼소진행간예.사갑기우담서염(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT)비색법검측세포활력,류식세포술검측세포주기,실시정량취합매련반응법분석세포Ⅰ형섬용매원격활제억제물( plasminogen activatorinhibitor-1,PAI-1)、전화생장인자β1(transforming growth factor-β1,TGF-β1)、뇨격매형섬용매원격활인자(urokinase-type plasminogen activator,uPA)화기질금속단백매2(matrix metalloproteinase-2,MMP-2)적기인표체.결과:MTT비색법검측표명단삼소가억제유200 μmol/L을철유도적HSC증식,류식세포술분석발현단삼소가사처우S기적세포수량명현증가(P＜0.05).차외,단삼소환능하조TGF-β1화PAI-1적기인표체,병증가uPA적전록수평(P＜0.01),단대MMP-2적표체몰유명현영향.결론:단삼소가억제을철유도적HSC-T6적활화여증식,병가조절삼여세포외기질침적적세포인자적표체.
OBJECTIVE:To evaluate the effects of danshensu,the main component of the extract of Chinese medicine Salvia miltiorrhiza,on the proliferation and activation of hepatic stellate cells ( HSCs).METHODS:The activation of HSC-T6 was induced by exposure to acetaldehyde.In the meantime,different doses of danshensu were added to the culture medium.After 24 h of treatment with danshensu in acetaldehyde,the viability of HSC-T6 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay,the cell cycle was determined through flow cytometry,and the gene transcription levels of plasminogen activator inhibitor-1 (PAI-1),transforming growth factor-β1 (TGF-β1),urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-2 (MMP-2) were analyzed by real-time quantitative polymerase chain reaction.RESULTS:The proliferation of HSCs induced by 200 μmol/L acetaldehyde could be significantly inhibited by danshensu,and the percentage of HSCs in S phase was significantly increased as compared with the control cells (P＜0.05),which were respectively evidenced by MTT assay and flow cytometry.Danshensu down-regulated the mRNA expression of TGF-β1 and PAI-1 and up-regulated the uPA transcription level (P＜0.01),while the transcription level of MMP-2 was not significantly affected in HSC-T6.CONCLUSION:Danshensu can inhibit the proliferation and activation of HSC-T6,as well as regulate some cytokines involved in extracellular matrix accumulation,which offers a potential therapeutic alternative for liver fibrosis.