中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
5期
322-324
,共3页
齐蔓莉%王敬%刘原君%刘全忠%陈锦英
齊蔓莉%王敬%劉原君%劉全忠%陳錦英
제만리%왕경%류원군%류전충%진금영
衣原体,沙眼%白细胞介素2%疫苗,DNA%免疫
衣原體,沙眼%白細胞介素2%疫苗,DNA%免疫
의원체,사안%백세포개소2%역묘,DNA%면역
Chlamydia,trachomatis%Interleukin 2%Vaccines,DNA%Immunity
目的 探讨小鼠白介素2重组质粒对沙眼衣原体E型DNA疫苗细胞免疫效果的影响.方法 以BALB/c小鼠为实验动物,采用肌内接种免疫的方式,将小鼠分为接种空质粒的阴性对照组、DNA疫苗免疫组、DNA疫苗联合小鼠白介素2重组质粒免疫组和接种灭活沙眼衣原体E型原体的阳性对照组,观察小鼠后足垫局部的肿胀情况、脾淋巴细胞增殖试验、血清IL-4和IFN-γ水平和同型沙眼衣原体生殖道攻击后局部的衣原体培养情况.结果 4组实验动物的脾淋巴细胞增殖指数分别为1.37±0.21、2.52±0.30、3.64±0.41、3.77±0.34,联合免疫组和阳性对照组比较差异无统计学意义,与另2组比较,差异有统计学意义.4组的血清IL-4分别为(25.37±18.93)、(24.75±8.49)、(21.74±6.43)、(38.49±12.24)pg/ml,灭活原体组高于其他各组；4个组的血清IFN-y分别为(310.8±160.7) pg/ml、(601.3±357.9) pg/ml、(1923.3±518.1) pg/ml、(2712.5±887.2)pg/m1,联合免疫组和阳性对照组相当,与另两组比较,差异有统计学意义.沙眼衣原体E型原体攻击后,空质粒组小鼠生殖道局部能够培养出衣原体；另3个组衣原体培养阴性.结论 白介素2基因佐剂能够增强沙眼衣原体E型DNA疫苗的细胞免疫效应,且以Th1型反应为主.
目的 探討小鼠白介素2重組質粒對沙眼衣原體E型DNA疫苗細胞免疫效果的影響.方法 以BALB/c小鼠為實驗動物,採用肌內接種免疫的方式,將小鼠分為接種空質粒的陰性對照組、DNA疫苗免疫組、DNA疫苗聯閤小鼠白介素2重組質粒免疫組和接種滅活沙眼衣原體E型原體的暘性對照組,觀察小鼠後足墊跼部的腫脹情況、脾淋巴細胞增殖試驗、血清IL-4和IFN-γ水平和同型沙眼衣原體生殖道攻擊後跼部的衣原體培養情況.結果 4組實驗動物的脾淋巴細胞增殖指數分彆為1.37±0.21、2.52±0.30、3.64±0.41、3.77±0.34,聯閤免疫組和暘性對照組比較差異無統計學意義,與另2組比較,差異有統計學意義.4組的血清IL-4分彆為(25.37±18.93)、(24.75±8.49)、(21.74±6.43)、(38.49±12.24)pg/ml,滅活原體組高于其他各組；4箇組的血清IFN-y分彆為(310.8±160.7) pg/ml、(601.3±357.9) pg/ml、(1923.3±518.1) pg/ml、(2712.5±887.2)pg/m1,聯閤免疫組和暘性對照組相噹,與另兩組比較,差異有統計學意義.沙眼衣原體E型原體攻擊後,空質粒組小鼠生殖道跼部能夠培養齣衣原體；另3箇組衣原體培養陰性.結論 白介素2基因佐劑能夠增彊沙眼衣原體E型DNA疫苗的細胞免疫效應,且以Th1型反應為主.
목적 탐토소서백개소2중조질립대사안의원체E형DNA역묘세포면역효과적영향.방법 이BALB/c소서위실험동물,채용기내접충면역적방식,장소서분위접충공질립적음성대조조、DNA역묘면역조、DNA역묘연합소서백개소2중조질립면역조화접충멸활사안의원체E형원체적양성대조조,관찰소서후족점국부적종창정황、비림파세포증식시험、혈청IL-4화IFN-γ수평화동형사안의원체생식도공격후국부적의원체배양정황.결과 4조실험동물적비림파세포증식지수분별위1.37±0.21、2.52±0.30、3.64±0.41、3.77±0.34,연합면역조화양성대조조비교차이무통계학의의,여령2조비교,차이유통계학의의.4조적혈청IL-4분별위(25.37±18.93)、(24.75±8.49)、(21.74±6.43)、(38.49±12.24)pg/ml,멸활원체조고우기타각조；4개조적혈청IFN-y분별위(310.8±160.7) pg/ml、(601.3±357.9) pg/ml、(1923.3±518.1) pg/ml、(2712.5±887.2)pg/m1,연합면역조화양성대조조상당,여령량조비교,차이유통계학의의.사안의원체E형원체공격후,공질립조소서생식도국부능구배양출의원체；령3개조의원체배양음성.결론 백개소2기인좌제능구증강사안의원체E형DNA역묘적세포면역효응,차이Th1형반응위주.
Objective To study the effect of a recombinant plasmid encoding mouse interleukin 2 (mlL-2) on the immunogenicity of DNA vaccine against Chlamydia trachomatis (Ct) serovar E.Methods BALB/c mice were divided into 4 groups to be intramuscularly inoculated with blank plasmid (negative control group),DNA vaccine against Ct serovar E (DNA vaccine group),DNA vaccine against Ct serovar E and a recombinant plasmid containing mIL-2 (combination group),and inactivated Ct serovar E elementary bodies (positive control group),respectively.The immunological effects were evaluated by posterior foot pad thickness,proliferation level of spleen lymphocytes,serum level of IL-4 and interferon (IFN)-γ in mice,and the capability to clear Ct genital tract infection.Results The proliferation index of spleen lymphocytes in the combination group and positive control group was similar (3.64 ± 0.41 vs.3.77 ± 0.34),but was significantly different from that in the blank control group and DNA vaccine group (1.37 ± 0.21 and 2.52 ± 0.30).The serum level of IL-4 was (38.49 ± 12.24) pg/ml in the positive control group,significantly higher than in the negative control group,DNA vaccine group and combination group ((25.37 ± 18.93),(24.75 ± 8.49),(21.74 ± 6.43) pg/ml,respectively).With respect to the serum level of IFN-γ,the combination group and positive control group were similar ((1923.3 ± 518.1) pg/ml vs.(2712.5 ± 887.2) pg/ml),but were significantly different from the negative control group and vaccine group ((310.8 ± 160.7) pg/ml and (601.3 ± 357.9) pg/ml).Six days after Ct challenge,the exfoliated cells from genital tract were positive for Ct culture in the negative control group,but negative in the other 3 groups.Conclusion IL-2 genetic adjuvant can enhance the immune response,especially Th1 type response,induced by the DNA vaccine against Ct serovar E.
