CAJ | 학술논문

目的检测T淋巴细胞中的11种CD4+T细胞因子在胆道闭锁(biliary atresia,BA)患儿肝脏组织中的表达,以探讨其在胆道闭锁发病机制中的意义.方法在病理的基础上,采用流式微球技术对29例BA及9例对照组患儿肝脏组织CD4+T细胞表达的11种细胞因子(IL-12p70、IFN-γ、IL-2、IL-10、IL-8、IL-6、IL-4、IL-5、IL-1β、TNF-α和TNF-β)同时进行定量检测,并对其代表因子IFN-γ进行免疫组化定位分析.结果 CD4+T细胞表达的11种细胞因子中,BA组患儿肝脏组织中IL-1β、IL-2、IL-6、IL-8、IFN-γ、TNF-α以及Th1细胞因子总量(IL-1β、IL-2、IL-8、IL-12p70、IFN-γ、TNF-α、TNF-β)与促炎因子总量(IL-1β、IL-2、IL-6、IL-8、IL-12p70、IFN-γ、TNF-α、TNF-β)分别为2920.69、1 106.01、152.22、12 614.22、834.18、161.29、19 504.55、19653.06,数值明显高于对照组的1 096.00、243.68、5.98、965.17、147.28、30.56、2 617.93、2 623.91,差异具有统计学意义(P＜0.05).结论由CD4+Th1细胞及其细胞因子所介导的针对胆道上皮细胞的免疫炎症性疾病可能是造成胆道闭锁的主要原因之一.
목적 검측T림파세포중적11충CD4+T세포인자재담도폐쇄(biliary atresia,BA)환인간장조직중적표체,이탐토기재담도폐쇄발병궤제중적의의.방법 재병리적기출상,채용류식미구기술대29례BA급9례대조조환인간장조직CD4+T세포표체적11충세포인자(IL-12p70、IFN-γ、IL-2、IL-10、IL-8、IL-6、IL-4、IL-5、IL-1β、TNF-α화TNF-β)동시진행정량검측,병대기대표인자IFN-γ진행면역조화정위분석.결과 CD4+T세포표체적11충세포인자중,BA조환인간장조직중IL-1β、IL-2、IL-6、IL-8、IFN-γ、TNF-α이급Th1세포인자총량(IL-1β、IL-2、IL-8、IL-12p70、IFN-γ、TNF-α、TNF-β)여촉염인자총량(IL-1β、IL-2、IL-6、IL-8、IL-12p70、IFN-γ、TNF-α、TNF-β)분별위2920.69、1 106.01、152.22、12 614.22、834.18、161.29、19 504.55、19653.06,수치명현고우대조조적1 096.00、243.68、5.98、965.17、147.28、30.56、2 617.93、2 623.91,차이구유통계학의의(P＜0.05).결론 유CD4+Th1세포급기세포인자소개도적침대담도상피세포적면역염증성질병가능시조성담도폐쇄적주요원인지일.
Objective To investigate the cytokines produced by CD4 + T cells in liver tissues of the patients with biliary atresia (BA). Methods The liver tissues of the 29 patients with BA were grounded and centrifuged. Then, the supernatants were collected. Nine patients not affected BA served as controls. The 11 cytokines produced by CD4 + T cells including IL- 12p70, IFN-γ, IL-2, IL- 10, IL-8,IL-6, IL-4, IL- 5, IL-1 β, TNF-α and TNF-β were detected using microsphere-based flow cytometry. Results Of the BA patients, the amount of IL-1β, IL-2, IL-6, IL-8, IFN-γ and TNF-α were 2 920. 69,1 106. 01,152. 22,12 614. 22,834. 18 and 161.29, respectively. The total amount of the Th1 cytokines including IL-1β, IL-2, IL-8, IL-12p70, IFN-γ, TNF-α and TNF-β was 19 504. The total amount of the proinflammatory cytokines including IL-1β, IL-2, IL-6, IL-8, IL-12p70, IFN-γ, TNFα and TNF-β was 19 653. 06. The amounts of these cytokines were significantly higher than those of the control group,which were 1 096.00,243.68,5.98,965.17,147. 28,30.56,2617.93, and 2 623.91, respectively. Conclusions The increased expressions of the cytokines expressed by the CD4 + Th1 cells may be one of the main causes of BA.