中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2011年
6期
741-743
,共3页
乌饭树叶%四氯化碳%肝损伤
烏飯樹葉%四氯化碳%肝損傷
오반수협%사록화탄%간손상
Vaccinium bracteatum thumb leaf%Carbon tetrachloride%Liver injury
目的 观察乌饭树叶醇提取物对四氯化碳致小鼠急性肝损伤的保护作用.方法 60只小鼠随机分为六组:正常对照组、模型组、联苯双酯组、乌饭树叶醇提取物低、中、高剂量组,用四氯化碳造成小鼠急性肝损伤模型,测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、肝组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量.肝组织常规HE染色观察.结果 急性肝损伤小鼠血清ALT、AST水平以及肝组织MDA含量显著升高,伴SOD活性明显下降,与正常对照组比较有显著差异.预先给予乌饭树叶醇提取物能显著改善小鼠肝损伤,降低血清ALT、AST水平以及肝组织MDA含量,并升高组织SOD活性.结论 乌饭树叶醇提取物对四氯化碳致小鼠急性肝损伤有明显保护作用,机制可能与其抗氧化效应和清除自由基的作用有关.
目的 觀察烏飯樹葉醇提取物對四氯化碳緻小鼠急性肝損傷的保護作用.方法 60隻小鼠隨機分為六組:正常對照組、模型組、聯苯雙酯組、烏飯樹葉醇提取物低、中、高劑量組,用四氯化碳造成小鼠急性肝損傷模型,測定血清穀丙轉氨酶(ALT)、穀草轉氨酶(AST)、肝組織超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量.肝組織常規HE染色觀察.結果 急性肝損傷小鼠血清ALT、AST水平以及肝組織MDA含量顯著升高,伴SOD活性明顯下降,與正常對照組比較有顯著差異.預先給予烏飯樹葉醇提取物能顯著改善小鼠肝損傷,降低血清ALT、AST水平以及肝組織MDA含量,併升高組織SOD活性.結論 烏飯樹葉醇提取物對四氯化碳緻小鼠急性肝損傷有明顯保護作用,機製可能與其抗氧化效應和清除自由基的作用有關.
목적 관찰오반수협순제취물대사록화탄치소서급성간손상적보호작용.방법 60지소서수궤분위륙조:정상대조조、모형조、련분쌍지조、오반수협순제취물저、중、고제량조,용사록화탄조성소서급성간손상모형,측정혈청곡병전안매(ALT)、곡초전안매(AST)、간조직초양화물기화매(SOD)활성、병이철(MDA)함량.간조직상규HE염색관찰.결과 급성간손상소서혈청ALT、AST수평이급간조직MDA함량현저승고,반SOD활성명현하강,여정상대조조비교유현저차이.예선급여오반수협순제취물능현저개선소서간손상,강저혈청ALT、AST수평이급간조직MDA함량,병승고조직SOD활성.결론 오반수협순제취물대사록화탄치소서급성간손상유명현보호작용,궤제가능여기항양화효응화청제자유기적작용유관.
Objective To investigate the protective effect of the ethanol extract of vacciniuin bracteatum thumb leaf on acute liver injury induced by CC14 in mice. Methods Sixty mice were allocated into 6 groups randomly, respectively as the normal control group,the model group,the positive control group,the ethanol extract low,middle and high dose group. Each group had 10 mice. Acute hepatic injury model of mice was made by using CC14. The level of ALT, AST in blood serum and SOD,MDA in hepatic tissue were observed. The hepatic tissue was HE-stained conventionally and observed. Results Acute liver injury in mice could be successfully induced by CC14 ,as demonstrated by increased serum ALT, AST levels and severe histological observations. Pretreatment with the ethanol extract of vaccinium bracteatum thumb leaf indicated its potent protective effects on acute liver injury, as demonstrated by a significant decrease in ALT, AST and MDA. Conclusion The ethanol extract of vaccinium bracteatum thumb leaf had an obviously protective effect on acute liver injury induced by CC14, which could be due to its antioxidative stress effect.