CAJ | 학술논문

目的构建人乳头瘤病毒11型L1/E7嵌合DNA疫苗pcDNA3 L1-E7并研究其在小鼠中诱导的免疫效应.方法用分子克隆技术构建重组真核表达质粒peDNA3 L1-E7.重组质粒DNA股四头肌注射免疫小鼠,用ELISA方法检测L1、E7特异性抗体和脾细胞分泌的IL-2、γ-INF;MTT法检测脾淋巴细胞增殖反应.结果成功构建pcDNA3 L1-E7.免疫小鼠后,重组质粒可诱导机体产生特异性脾淋巴细胞增殖及IL-2、γ-INF分泌增加,并诱导机体产生HPV 11-E7 IgG和HPV 11-L1 IgG抗体.结论嵌合DNA疫苗pcDNA3 L1-E7能诱导小鼠产生特异性的细胞免疫和体液免疫反应.
목적 구건인유두류병독11형L1/E7감합DNA역묘pcDNA3 L1-E7병연구기재소서중유도적면역효응.방법 용분자극륭기술구건중조진핵표체질립peDNA3 L1-E7.중조질립DNA고사두기주사면역소서,용ELISA방법 검측L1、E7특이성항체화비세포분비적IL-2、γ-INF;MTT법검측비림파세포증식반응.결과 성공구건pcDNA3 L1-E7.면역소서후,중조질립가유도궤체산생특이성비림파세포증식급IL-2、γ-INF분비증가,병유도궤체산생HPV 11-E7 IgG화HPV 11-L1 IgG항체.결론 감합DNA역묘pcDNA3 L1-E7능유도소서산생특이성적세포면역화체액면역반응.
Objective To construct chimerical DNA vaccine plasmid of human papiUomavirus type 11 (HPV11) L1-E7, and to evaluate its immunogenicity. Methods Molecular cloning techniques were used to construct recombinant plasmid PeDNA3 L1-E7 as a DNA vaccine. BALB/c mice were vaccinated with DNA recombinants through muscle injection. IL-2 and γ-INF secreted by immunized spleens lymphocyte and HPV 11 LI or E7 specific antibodies were assayed by ELISA method. Spleens lymphocyte proliferation was measured by MTT assay. Results The chimerical DNA plasmid of pcDNA3 LI-E7 was constructed correctly. Specific anti-HPV11 E7 and L1 antibodies, specific lymphocyte proliferation and secretions of IL-2 and γ-INF were detected in vaccinated mice. Conclusion Specific immune response, including cellular immunity and humoral immunity, could been detected in mice vaccinated with chimerical DNA vaccine of pcDNA3 L1-E7.