CAJ | 학술논문

目的:探讨不同剂量蛇毒抗高凝状态酶(AHCSE)对小鼠肝癌H22生长作用的影响.方法:小鼠皮下接种小鼠肝癌(H22)后,待肿瘤长成 10 mm2 分别经腹腔注射不同剂量AHCSE,每天1次,连续 10 d,观察瘤重抑制率、瘤体积、抑瘤率、脾指数和白细胞及其组成成分,统计采用LDS-t,检验水准α=0.05.采用白细胞及其组分与脾指数的相关和回归.结果:中、高剂量组AHCSE具有明显的抗肿瘤作用,而低剂量组抗肿瘤作用效果不佳,但高剂量组的小鼠体重降低明显.实验组的脾系数明显高于对照组,并且随着AHCSE的剂量增大脾系数也增大.白细胞随着AHCSE剂量的增大而增大,白细胞及其组分与脾指数存在相关性和回归直线.结论:在一定的范围内,随着AHCSE的剂量增大,抗肿瘤效果增强,但副作用也增大,其机制可能与凋亡和激活免疫系统有关.
목적:탐토불동제량사독항고응상태매(AHCSE)대소서간암H22생장작용적영향.방법:소서피하접충소서간암(H22)후,대종류장성 10 mm2 분별경복강주사불동제량AHCSE,매천1차,련속 10 d,관찰류중억제솔、류체적、억류솔、비지수화백세포급기조성성분,통계채용LDS-t,검험수준α=0.05.채용백세포급기조분여비지수적상관화회귀.결과:중、고제량조AHCSE구유명현적항종류작용,이저제량조항종류작용효과불가,단고제량조적소서체중강저명현.실험조적비계수명현고우대조조,병차수착AHCSE적제량증대비계수야증대.백세포수착AHCSE제량적증대이증대,백세포급기조분여비지수존재상관성화회귀직선.결론:재일정적범위내,수착AHCSE적제량증대,항종류효과증강,단부작용야증대,기궤제가능여조망화격활면역계통유관.
AIM:To research the inhibitory effect of antihypercoagulability state enzyme (AHCSE) of snake venom in different dosages in mice with Hepatocarcinoma. METHODS: Hepatic cancer H22 model in mice was performed through subcutaneous inoculation of Right-Sidedness Axilla of BALB/C mice. Different dosages of AHCSE (1, 2, and 4 mg*kg-1) were injected via abdominal cavity everyday for 10 days. When the tumor grew to 10 mm3, the weight, volume, percent inhibition of tumor, spleen index and the number and the component of white blood cells were observed. RESULTS: The middle and high dosages of AHCSE had obvious inhibition in hepatic cancer. It showed a significant dose-dependent relation in the effects of AHCSE on neoplastic weight, neoplastic volume and splenic index. There was a linear regression between the number of leucocytes and the splenic index. CONCLUSION: AHCSE of 2-4 mg*kg-1 has significant inhibitory effect on tumor, and shows dose-related effect in mice with Hepatocarcinoma. Its mechanism is presumably associated with activating immune system.