CAJ | 학술논문

以西黄松成熟胚为外植体诱导不定芽,在GD+9.85～19.70 μmol·L'-1 6-BA+0.0～14.42 μmol·L-1 NAA上不定芽诱导率最高达65.8%,平均增殖率为7,最大增殖率达10;不定芽形成有2种途径,即子叶直接形成不定芽和子叶组织再分化形成不定芽;NAA不利于外植体不定芽的诱导;不定芽的生长和扩繁采用不加生长调节剂的1/2 GD和1/2 SH培养基;培养基中加入适量的活性炭有利于不定芽和根的生长.不定嫩梢在1/2 GD和1/2 SH附加不同浓度NAA和GA,3的培养基上进行生根诱导,试验结果表明:NAA对不定根的形成起主要作用,在1/2 GD+28.84 μmol·L'-1 NAA+4.17 μmol·L'-1 GA,3培养基中不定梢的生根率为16.7%.在离体培养条件下,以西黄松成熟胚为外植体获得了再生植株.
이서황송성숙배위외식체유도불정아,재GD+9.85～19.70 μmol·L'-1 6-BA+0.0～14.42 μmol·L-1 NAA상불정아유도솔최고체65.8%,평균증식솔위7,최대증식솔체10;불정아형성유2충도경,즉자협직접형성불정아화자협조직재분화형성불정아;NAA불리우외식체불정아적유도;불정아적생장화확번채용불가생장조절제적1/2 GD화1/2 SH배양기;배양기중가입괄량적활성탄유리우불정아화근적생장.불정눈소재1/2 GD화1/2 SH부가불동농도NAA화GA,3적배양기상진행생근유도,시험결과표명:NAA대불정근적형성기주요작용,재1/2 GD+28.84 μmol·L'-1 NAA+4.17 μmol·L'-1 GA,3배양기중불정소적생근솔위16.7%.재리체배양조건하,이서황송성숙배위외식체획득료재생식주.
Adventitious buds were induced from the excised mature zygotic embryos of Ponderosa Pine (Pinus ponderosa). Thehighest induction rate (65.8%) of adventitious buds was obtained with 10-fold multiplication at the highest and 7-foldformation of adventitious buds. Buds proliferation and elongation were achieved on 1/2GD and 1/2SH without growth regulators.A proper amount of activated charcoal promoted adventitious buds elongation and root' growth. The adventitious shoots ofPonderosa Pine were used to induce adventitious roots on 1/2GD and 1/2SH media supplemented with different concentrations ofNAA and GA<,3> . The results showed that NAA played a determinative role in initiating the adventitious roots. Sixteen point sevenpaper, the experimental results showed that roots were successfully induced from cultured mature zygotic embryos of PonderosaPine.