中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2009年
12期
32-34
,共3页
车前子%槲皮素%木犀草素%山柰酚%芹菜素%含量测定%反相高效液相色谱法
車前子%槲皮素%木犀草素%山柰酚%芹菜素%含量測定%反相高效液相色譜法
차전자%곡피소%목서초소%산내분%근채소%함량측정%반상고효액상색보법
目的 建立车前子的槲皮素、山柰酚、木犀草素、芹菜素含量测定方法.方法 采用反相高效液相色谱(RP-HPLC)法.色谱柱为Zorbax SB-C18柱(150 mm×4.6 mm,5μm);检测波长360 mn,流动相为甲醇-0.2%磷酸(45:55),柱温30℃,流速1.0 mL/min.结果 槲皮素、山亲酚、木犀草素、芹菜素的回归方程分别为Y=1 504.412X+9.975 6,Y=1 991.745 X+8.605 1,Y=567.591X+2.539 7,Y=1 811.803X+0.307 4;质量浓度线性范围分别是0.055 22～0.193 3μg/mL,0.046 1～0.161 3 μg/mL,0.125 5～0.439 3 μg/mL,0.060 3～0.211μg/mL,r在0.999 9～1.000 0之间;槲皮素、山柰酚的加样回收率分别为92.87%和102.95%,RSD分别为0.28%和1.08%;样品分别含槲皮素、山柰酚、木犀草素、芹菜素0.81,0.634,0,0.08 mg/g.结论 RP-HPLC法分析车前予中黄酮化合物的含量,方法简便可行,重复性好,结果可靠.
目的 建立車前子的槲皮素、山柰酚、木犀草素、芹菜素含量測定方法.方法 採用反相高效液相色譜(RP-HPLC)法.色譜柱為Zorbax SB-C18柱(150 mm×4.6 mm,5μm);檢測波長360 mn,流動相為甲醇-0.2%燐痠(45:55),柱溫30℃,流速1.0 mL/min.結果 槲皮素、山親酚、木犀草素、芹菜素的迴歸方程分彆為Y=1 504.412X+9.975 6,Y=1 991.745 X+8.605 1,Y=567.591X+2.539 7,Y=1 811.803X+0.307 4;質量濃度線性範圍分彆是0.055 22～0.193 3μg/mL,0.046 1～0.161 3 μg/mL,0.125 5～0.439 3 μg/mL,0.060 3～0.211μg/mL,r在0.999 9～1.000 0之間;槲皮素、山柰酚的加樣迴收率分彆為92.87%和102.95%,RSD分彆為0.28%和1.08%;樣品分彆含槲皮素、山柰酚、木犀草素、芹菜素0.81,0.634,0,0.08 mg/g.結論 RP-HPLC法分析車前予中黃酮化閤物的含量,方法簡便可行,重複性好,結果可靠.
목적 건립차전자적곡피소、산내분、목서초소、근채소함량측정방법.방법 채용반상고효액상색보(RP-HPLC)법.색보주위Zorbax SB-C18주(150 mm×4.6 mm,5μm);검측파장360 mn,류동상위갑순-0.2%린산(45:55),주온30℃,류속1.0 mL/min.결과 곡피소、산친분、목서초소、근채소적회귀방정분별위Y=1 504.412X+9.975 6,Y=1 991.745 X+8.605 1,Y=567.591X+2.539 7,Y=1 811.803X+0.307 4;질량농도선성범위분별시0.055 22～0.193 3μg/mL,0.046 1～0.161 3 μg/mL,0.125 5～0.439 3 μg/mL,0.060 3～0.211μg/mL,r재0.999 9～1.000 0지간;곡피소、산내분적가양회수솔분별위92.87%화102.95%,RSD분별위0.28%화1.08%;양품분별함곡피소、산내분、목서초소、근채소0.81,0.634,0,0.08 mg/g.결론 RP-HPLC법분석차전여중황동화합물적함량,방법간편가행,중복성호,결과가고.
