贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2009年
10期
139-141
,共3页
李川%杨明境%姚俊杰%安苗%何登菊
李川%楊明境%姚俊傑%安苗%何登菊
리천%양명경%요준걸%안묘%하등국
大鳍鳠%染色体%核型
大鰭鳠%染色體%覈型
대기화%염색체%핵형
Mystus macropterus%chromosome%karyotype
为了认识大鳍鳠的染色体组特征,为大鳍鳗的遗传多样性积累数据,同时为其驯化繁殖提供技术资料,采用PHA、秋水仙素腹腔注射,肾细胞直接制片法分析了大鳍鳗染色体的核型.结果表明:大鳍鳗二倍体染色体数目2n=56,核型公式为2n=16m+14 sm+12 st+14t,其染色体总臂数NF=86.
為瞭認識大鰭鳠的染色體組特徵,為大鰭鰻的遺傳多樣性積纍數據,同時為其馴化繁殖提供技術資料,採用PHA、鞦水仙素腹腔註射,腎細胞直接製片法分析瞭大鰭鰻染色體的覈型.結果錶明:大鰭鰻二倍體染色體數目2n=56,覈型公式為2n=16m+14 sm+12 st+14t,其染色體總臂數NF=86.
위료인식대기화적염색체조특정,위대기만적유전다양성적루수거,동시위기순화번식제공기술자료,채용PHA、추수선소복강주사,신세포직접제편법분석료대기만염색체적핵형.결과표명:대기만이배체염색체수목2n=56,핵형공식위2n=16m+14 sm+12 st+14t,기염색체총비수NF=86.
Intraperitoneally injection of PHA and colchicine, kidney cells air-drying method were applied to analyze the karyotype of Mystus macropterus to study the genome characteristics of M. macropterus, also to accumulate its genetic diversity data, and to provide technical information to its domestication and reproduction. The results showed that the chromosome number of M. macropterus was 2n=56, the formula of karyotype was 2n=16m+ 14sm+12st+14t, and the total number of chromosome arms were NF=86.