CAJ | 학술논문

[目的] 检测罗汉果茎尖玻璃化法超低温保存后再生植株的遗传稳定性.[方法] 对罗汉果玻璃化法超低温保存前后的植株进行过氧化物酶(POD)和酯酶(EST)同工酶酶谱分析、染色体计数和随机扩增多态性DNA(RAPD)分子标记.[结果] 超低温保存前后,POD同工酶图谱上均显示9条酶带,且迁移率一致;EST同工酶图谱上均显示8条酶带,迁移率也一致;POD和EST同工酶谱、染色体数目和RAPD谱带均未发现差异.[结论] 玻璃化法超低温保存罗汉果种质资源安全、稳定.
[목적] 검측라한과경첨파리화법초저온보존후재생식주적유전은정성.[방법] 대라한과파리화법초저온보존전후적식주진행과양화물매(POD)화지매(EST)동공매매보분석、염색체계수화수궤확증다태성DNA(RAPD)분자표기.[결과] 초저온보존전후,POD동공매도보상균현시9조매대,차천이솔일치;EST동공매도보상균현시8조매대,천이솔야일치;POD화EST동공매보、염색체수목화RAPD보대균미발현차이.[결론] 파리화법초저온보존라한과충질자원안전、은정.
[Objective] The research aimed to examine the genetic stability of regenerated plants from Siraitia grosvenorii shoot-tips after cryopreservation by vitrification method.[Method] Plants before cryopreservation and those regenerated from Siraitia grosvenorii shoot-tips after cryopreservation by vitrification were examined by peroxidase and esterase isoenzyme analysis.The number of chromosomes was counted and the molecule markers of random amplified polymorphic DNA (RAPD) was made.[Result] Before and after cryopreservation,there were 9 bands on POD isoenzyme patterns and there were 8 bands on EST isoenzyme patterns,with the same mobility.There were no variations before and after cryopreservation at peroxidase and esterase isozyme patterns,the number of chromosomes and RAPD patterns.[Conclusion] Cryopreservation of S.grosvenorii germplasm resources by vitrification was safe and stable.