CAJ | 학술논문

目的了解北京市5岁以下婴幼儿轮状病毒腹泻患者的流行特点.方法 2011年1月至12月在北京市3家医院肠道门诊收集输液两次以上5岁以下腹泻患者病例604例,采集粪便标本,应用酶联免疫吸附试验检测轮状病毒抗原,阳性标本用半巢式反转录PCR进行基因分型.结果 604例标本中96例轮状病毒抗原阳性,阳性率为15.89％.秋冬季为A组轮状病毒腹泻的发病高峰,其中11月份A组轮状病毒阳性率最高(55.56％,30/54).轮状病毒腹泻主要发生于3～23月龄患者(93.76％,90/96).对轮状病毒抗原阳性标本进行G/P分型,G分型以G3 +G9型混合感染占第一位( 37.50％,36/96),其它型别主要有G3型(28.12％,27/96),G1型(11.46％,11/96),G9型(9.38％,9/96),G2型(8.33％,8/96).P分型以P8型为主要优势株(86.46％,83/604),其次为P4型( 10.42％,10/96).最常见的G/P基因型组合为G3 +G9/[P8] (36.46％,35/96),其次为G3/[P8] ( 26.04％,25/96),G1/[ P8] (11.46％,11/96),G9/[ P8] (9.38％,9/96),G2/[P4](8.33％,8/96).结论 A组轮状病毒是北京市婴幼儿腹泻的主要病原之一,2011年主要流行株基因型组合为G3+ G9/P[8].
목적 료해북경시5세이하영유인륜상병독복사환자적류행특점.방법 2011년1월지12월재북경시3가의원장도문진수집수액량차이상5세이하복사환자병례604례,채집분편표본,응용매련면역흡부시험검측륜상병독항원,양성표본용반소식반전록PCR진행기인분형.결과 604례표본중96례륜상병독항원양성,양성솔위15.89％.추동계위A조륜상병독복사적발병고봉,기중11월빈A조륜상병독양성솔최고(55.56％,30/54).륜상병독복사주요발생우3～23월령환자(93.76％,90/96).대륜상병독항원양성표본진행G/P분형,G분형이G3 +G9형혼합감염점제일위( 37.50％,36/96),기타형별주요유G3형(28.12％,27/96),G1형(11.46％,11/96),G9형(9.38％,9/96),G2형(8.33％,8/96).P분형이P8형위주요우세주(86.46％,83/604),기차위P4형( 10.42％,10/96).최상견적G/P기인형조합위G3 +G9/[P8] (36.46％,35/96),기차위G3/[P8] ( 26.04％,25/96),G1/[ P8] (11.46％,11/96),G9/[ P8] (9.38％,9/96),G2/[P4](8.33％,8/96).결론 A조륜상병독시북경시영유인복사적주요병원지일,2011년주요류행주기인형조합위G3+ G9/P[8].
Objective To investigate the epidemiological characteristics of group A rotavirus diarrhea in children under 5 years old in Beijing.Methods Stool specimens of 604 children with diarrhea,who have got infusion twice and more,were collected from three hospitals in Beijing throughout 2011.The specimens were detected by ELISA,and the positive samples were genotyped using semi-nested reverse transcription PC R.Results A total of 96 positive cases were detected among 604 patients with diarrhea,with the positive rate of 15.89％.Group A rotavirus diarrhea was most common during autumn and winter,and the highest positive rate was in November (55.56％,30/54).Rotavirus diarrhea most occurred in 3 ～ 23-month-old infants ( 93.76％,90/96 ).All the positive specimens were genotyped for G/P.G3 and G9 mixed infection rank first (37.50％,36/96),followed by G3 (28.12％,27/96),Gl (11.46％,11/96),G9 (9.38％,9/96),G2 (8.33％,8/96).P8 type was the most dominant (86.46％,83/604),and the second was P4 (10.42％,10/96).G3 + G9/P [ 8 ] was the most common G/P combination (36.46％,35/96),followed by G3/[P8] (26.04％,25/96),G1/[P8] (11.46％,11/96),G9/[P8] (9.38％,9/96),G2/[P4] (8.33％,8/96).Conclusion Rotavirus was one of the main pathogens for infantile diarrhea,and the major epidemic strains was G3 + G9/P[ 8 ].