CAJ | 학술논문

目的观察异氟醚对老年小鼠空间学习记忆能力及其大脑Caspase-3蛋白表达、细胞凋亡的影响.方法 24只16月龄C57BL/6老年小鼠分层随机分为异氟醚组(Iso,n=12)和对照组(Con,n=12).lso组使用1.0%异氟醚行麻醉暴露4h/d,连续2d;Con组同期只使用30%的氧气和70%氮气混合气.在麻醉暴露后,采用Morris水迷宫检测小鼠空间学习记忆能力,再分别使用免疫荧光染色法及TUNEL法检测小鼠大脑皮质区(CX)、海马Cal区和齿状回(DG)的Caspase-3蛋白表达及神经细胞凋亡的变化.结果在Morris水迷宫的定位航行测试中,2组间逃避潜伏期的变化差异无显著性(F=0.007,P=1.235),但在空间探索实验测试中,Iso组在原平台所在象限(靶象限)停留时间[(34.5±5.0)%]明显短于Con组[(45.1±4.9)%],差异具有显著性(P<0.01).测试过程中2组小鼠寻找平台游泳的速度差异无显著性F=1.537,P=0.241).免疫荧光染色检测结果显示,DG、CAI及Cx区均可见Caspase-3阳性细胞,然而2组间的单位面积的阳性细胞数(阳性密度)比较差异无显著性(P>0.05).TUNEL 法检测到极少量阳性凋亡细胞.结论重复使用1%异氟醚麻醉暴露可损害老年小鼠的空间参考记忆能力,但无明显影响大脑Caspase-3蛋白表达及细胞凋亡.
목적 관찰이불미대노년소서공간학습기억능력급기대뇌Caspase-3단백표체、세포조망적영향.방법 24지16월령C57BL/6노년소서분층수궤분위이불미조(Iso,n=12)화대조조(Con,n=12).lso조사용1.0%이불미행마취폭로4h/d,련속2d;Con조동기지사용30%적양기화70%담기혼합기.재마취폭로후,채용Morris수미궁검측소서공간학습기억능력,재분별사용면역형광염색법급TUNEL법검측소서대뇌피질구(CX)、해마Cal구화치상회(DG)적Caspase-3단백표체급신경세포조망적변화.결과 재Morris수미궁적정위항행측시중,2조간도피잠복기적변화차이무현저성(F=0.007,P=1.235),단재공간탐색실험측시중,Iso조재원평태소재상한(파상한)정류시간[(34.5±5.0)%]명현단우Con조[(45.1±4.9)%],차이구유현저성(P<0.01).측시과정중2조소서심조평태유영적속도차이무현저성F=1.537,P=0.241).면역형광염색검측결과현시,DG、CAI급Cx구균가견Caspase-3양성세포,연이2조간적단위면적적양성세포수(양성밀도)비교차이무현저성(P>0.05).TUNEL 법검측도겁소량양성조망세포.결론 중복사용1%이불미마취폭로가손해노년소서적공간삼고기억능력,단무명현영향대뇌Caspase-3단백표체급세포조망.
Objective To investigate the effect of isoflurane on the spatial learning and memory in aged mice,and whether this is associated with the changes of Caspase-3 expression and apoptosis in brain.Methods Twenty-four CO57BL/6 aged mice(16 months)were randomly divided into isoflurane treatment group(Iso Group,n=12) and sham control group (Con Group,n=12).Mice in Iso group were exposed to 1% isoflurane in carrying gas of 30% oxygen,balance nitrogen in a warmed,humidified chamber for4 h per day for2 days.For Con group,animals were treated at the same condition with only carrying gas.After anesthetic exposures,behavioral testing was performed using the Morris water maze(MWM),and then changes of Caspase-3 expression and apoptosis in hippocampus CAI,dentate gyrus(DG) and cortex(CX) in brain were determined by using immunofluorecence staining and TUNEL staining.Results In hidden-platform training of MWM,the mean escape latency to platform showed no significant difference between the two groups (F=0.007,P=1.235),but the mice in Iso group showed obviously impaired retention of memory by spending more percentage of time swimming in the probe quadrant as compared to the control animals in the probe test((34.5±5.0)%vs(45.1±4.9)%.P<0.01).There was no significant difference in average swimming speed during the MWM testing trials between the two groups (F=1.537,P=0.241).A few Caspase-3 and apoptosis positive cells were found in hippocampus Cal,DG and CX regious,while no difference was found in the density of positive cells between the two groups(P>0.05).Conclusion 1% isoflurane repeatedly exposure significantly impaires the spatial reference memory in aged mouses,however does not significantly change the expression of easpase-3 and apoptosis in brain.