CAJ | 학술논문

目的探讨七氟醚对小鼠脑干细胞外信号调节激酶(ERK1/ERK2)磷酸化的影响,筛查脑干中与麻醉效应有关的核团.方法 BALB/c小鼠60只,8周龄,体重20～25 g,随机分为5组(n=12),对照组(Con组)无麻醉处理;Sevo-1组七氟醚麻醉5 min;Sevo-2组七氟醚麻醉1 h;E-1组七氟醚麻醉1 h,洗脱 2 min;E-2组七氟醚麻醉1 h,洗脱1 h.各组麻醉结束后处死小鼠,取6只小鼠,制备脑干组织切片,采用Westem blot法测定脑干ERK1/ERK2和磷酸化ERK1/ERK2(p-ERK1/ERK2)的表达;取6只小鼠,采用免疫组化法测定脑干不同核团p-ERK1/ERK2的表达.结果各组脑干ERK1/ERK2表达差异无统计学意义(P＞0.05).与Con组相比,其余4组脑干p-ERK1/ERK2表达水平升高(P＜0.01),而4组间差异无统计学意义(P＞0.05).光镜下可见孤束核(Sol)、延髓腹外侧网状核(LRt)、臂旁外侧核背侧亚核(LPBD)、中脑室周灰质腹外侧核(vLPAG)和动眼神经副核(EW)中有p-ERK1/ERK2表达.与Con组比较,Sevo-1组、Sevo.2组和E-1组LRt、Sol、EW、LPBD的p-ERK1/ERK2表达升高,vLPAG的P-ERK1/ERK2表达降低,E-2组LPBD的p-ERK1/ERK2表达升高(P＜0.05或0.01).与Sevo-2组比较,Sevo-1组和E-1组LPBD及E-2组LRt、Sol、EW、LPBD的p-ERK1/ERK2表达降低,E-2组vLPAG的p-ERK1/ERK2表达升高(P＜0.05或0.01).结论七氟醚麻醉时脑干核团p-ERK1/ERK2的改变可能参与了其全麻效应的中枢机制.
목적 탐토칠불미대소서뇌간세포외신호조절격매(ERK1/ERK2)린산화적영향,사사뇌간중여마취효응유관적핵단.방법 BALB/c소서60지,8주령,체중20～25 g,수궤분위5조(n=12),대조조(Con조)무마취처리;Sevo-1조칠불미마취5 min;Sevo-2조칠불미마취1 h;E-1조칠불미마취1 h,세탈 2 min;E-2조칠불미마취1 h,세탈1 h.각조마취결속후처사소서,취6지소서,제비뇌간조직절편,채용Westem blot법측정뇌간ERK1/ERK2화린산화ERK1/ERK2(p-ERK1/ERK2)적표체;취6지소서,채용면역조화법측정뇌간불동핵단p-ERK1/ERK2적표체.결과 각조뇌간ERK1/ERK2표체차이무통계학의의(P＞0.05).여Con조상비,기여4조뇌간p-ERK1/ERK2표체수평승고(P＜0.01),이4조간차이무통계학의의(P＞0.05).광경하가견고속핵(Sol)、연수복외측망상핵(LRt)、비방외측핵배측아핵(LPBD)、중뇌실주회질복외측핵(vLPAG)화동안신경부핵(EW)중유p-ERK1/ERK2표체.여Con조비교,Sevo-1조、Sevo.2조화E-1조LRt、Sol、EW、LPBD적p-ERK1/ERK2표체승고,vLPAG적P-ERK1/ERK2표체강저,E-2조LPBD적p-ERK1/ERK2표체승고(P＜0.05혹0.01).여Sevo-2조비교,Sevo-1조화E-1조LPBD급E-2조LRt、Sol、EW、LPBD적p-ERK1/ERK2표체강저,E-2조vLPAG적p-ERK1/ERK2표체승고(P＜0.05혹0.01).결론 칠불미마취시뇌간핵단p-ERK1/ERK2적개변가능삼여료기전마효응적중추궤제.