中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
2期
155-158
,共4页
杨慧%梁肇海%刘小立%王峰
楊慧%樑肇海%劉小立%王峰
양혜%량조해%류소립%왕봉
肺结核病%白细胞介素-10%干扰素-γ%基因多态性
肺結覈病%白細胞介素-10%榦擾素-γ%基因多態性
폐결핵병%백세포개소-10%간우소-γ%기인다태성
Pulmonary tuberculosis%Interleukin-10%Interferon-γ%Gene polymorphism
目的 探讨白介素-10(IL-10)基因-1082G/A位点和干扰素-γ(IFN-γ)基因+874A/T位点单核苷酸多态性(SNP)与肺结核病易感性的关系.方法 采用序列特异性引物PCR(PCR-SSP)及测序技术检测深圳地区汉族人群肺结核病例组及对照组各200例IL-10启动子区-1082G/A、IFN-γ+874A/T位点基因多态性.结果 病例组IL-10(-1082)位点A/A纯合子、A/G杂合子、G/G纯合子基因型频率分别为85.4%、13.1%、1.5%,对照组分别为77.5%、22.0%、0.5%;病例组A、G等位基因频率分别为91.9%、8.1%,对照组分别为88.5%、11.5%.两组间基因型分布差异有统计学意义(P＜0.05).病例组A/A基因型高于对照组,G/A基冈型低于对照组(P＜0.05).但两组的A、G等位基冈频率分布差异没有统计学意义.IFN-γ(+874)位点基因型频率及等位基因频率在病例组和对照组中分布无明显差异(P＞0.05).结论 中国汉族人群中细胞因子IL-10(-1082)基因SNP与肺结核易感性相关.A/A基因型可能是一个肺结核易感的风险因素.细胞因子IFN-γ(+874)基因位点SNP与肺结核易感性无关.
目的 探討白介素-10(IL-10)基因-1082G/A位點和榦擾素-γ(IFN-γ)基因+874A/T位點單覈苷痠多態性(SNP)與肺結覈病易感性的關繫.方法 採用序列特異性引物PCR(PCR-SSP)及測序技術檢測深圳地區漢族人群肺結覈病例組及對照組各200例IL-10啟動子區-1082G/A、IFN-γ+874A/T位點基因多態性.結果 病例組IL-10(-1082)位點A/A純閤子、A/G雜閤子、G/G純閤子基因型頻率分彆為85.4%、13.1%、1.5%,對照組分彆為77.5%、22.0%、0.5%;病例組A、G等位基因頻率分彆為91.9%、8.1%,對照組分彆為88.5%、11.5%.兩組間基因型分佈差異有統計學意義(P＜0.05).病例組A/A基因型高于對照組,G/A基岡型低于對照組(P＜0.05).但兩組的A、G等位基岡頻率分佈差異沒有統計學意義.IFN-γ(+874)位點基因型頻率及等位基因頻率在病例組和對照組中分佈無明顯差異(P＞0.05).結論 中國漢族人群中細胞因子IL-10(-1082)基因SNP與肺結覈易感性相關.A/A基因型可能是一箇肺結覈易感的風險因素.細胞因子IFN-γ(+874)基因位點SNP與肺結覈易感性無關.
목적 탐토백개소-10(IL-10)기인-1082G/A위점화간우소-γ(IFN-γ)기인+874A/T위점단핵감산다태성(SNP)여폐결핵병역감성적관계.방법 채용서렬특이성인물PCR(PCR-SSP)급측서기술검측심수지구한족인군폐결핵병례조급대조조각200례IL-10계동자구-1082G/A、IFN-γ+874A/T위점기인다태성.결과 병례조IL-10(-1082)위점A/A순합자、A/G잡합자、G/G순합자기인형빈솔분별위85.4%、13.1%、1.5%,대조조분별위77.5%、22.0%、0.5%;병례조A、G등위기인빈솔분별위91.9%、8.1%,대조조분별위88.5%、11.5%.량조간기인형분포차이유통계학의의(P＜0.05).병례조A/A기인형고우대조조,G/A기강형저우대조조(P＜0.05).단량조적A、G등위기강빈솔분포차이몰유통계학의의.IFN-γ(+874)위점기인형빈솔급등위기인빈솔재병례조화대조조중분포무명현차이(P＞0.05).결론 중국한족인군중세포인자IL-10(-1082)기인SNP여폐결핵역감성상관.A/A기인형가능시일개폐결핵역감적풍험인소.세포인자IFN-γ(+874)기인위점SNP여폐결핵역감성무관.
Objective To investigate the association of IL-10,IFN-γ gene polymorphisms at position-1082G/A and + 874A/T and susceptibility to pulmonary tuberculosis(PTB)through a case-control study.Methods Polymerase chain reaction with sequence specific primer(PCR-SSP)/sequencing method,single nucleotide polymorphisms(SNP)of IL-10 and IFN-γ gene at position-1082 and +874 were used on pulmonary tuberculosis patients(group PTB)and normal healthy subjects(group NHS)in the Han population from Shenzhen city of China.Results The genotype frequencies of A/A homozygotes,A/G heterozygous and G/G homozygous at-1082 site of IL-10 gene in patients with pulmonary tuberculosis were 85.4%,13.1%,1.5% respectively and A,G allele frequencies were 91.9%,8.1% respectively.In healthy subjects,the genotype frequencies of A/A homozygotes,A/G heterozygous and G/G homozygous were 77.5%,22.0%,0.5% respectively and A,G allele frequencies were 88.5%,11.5% respectively.Genotype distribution between the two groups was significantly different(P＜0.05).The genotype frequencies of A/A of IL-10 in the patient group were higher than that of control group.In contrast,the genotype frequencies of G/A in the control group were higher than that in the patient group(P＜0.05).There was no statistical difference found between A and G allele(P＞0.05).For IFN-γ(+874)and no significant difference was found on the distribution of genotypes or alleles between the two groups.Conclusion The IL-10(-1082)gene SNP might be associated with the susceptibility to tuberculosis.A/A genotype might be a risk factor for the susceptibility on tuberculosis while the SNP of cytokines(+874)might not be associated with the susceptibility to tuberculosis in Chinese Han populations.
