CAJ | 학술논문

目的探讨成年大鼠眶下神经束注射阿霉素对三叉神经脊束核尾侧区内P物质的影响.方法用0.5%的阿霉素溶液直接注射于一侧眶下神经束,对侧注射生理盐水作自身对照,分别在给药后3 d、1周、2周、3周、4周、5周、6周在光镜和电镜下观察双侧三叉神经节的变化;并用免疫组化方法观测三叉神经尾侧脊束核内(CNV)P物质(SP)免疫阳性颗粒的灰度值.结果阿霉素给药后,电子显微镜下观察到实验侧三叉神经节细胞形态改变,胞内出现大量空泡,神经纤维髓鞘发生变性、弯曲、增厚、分离;实验侧三叉神经尾侧脊束核内(CNV)P物质(SP)免疫阳性颗粒灰度值明显高于对照侧(P<0.01),不同时间组之间SP含量差异无统计学意义.结论眶下神经束注射阿霉素能够选择性破坏相应的节细胞,减少CNV区SP含量.SP作为一种重要的神经递质,其合成或释放异常可能与三叉神经痛的发病有关,其在三叉神经痛的发作机制中具有重要的作用.
목적 탐토성년대서광하신경속주사아매소대삼차신경척속핵미측구내P물질적영향.방법 용0.5%적아매소용액직접주사우일측광하신경속,대측주사생리염수작자신대조,분별재급약후3 d、1주、2주、3주、4주、5주、6주재광경화전경하관찰쌍측삼차신경절적변화;병용면역조화방법관측삼차신경미측척속핵내(CNV)P물질(SP)면역양성과립적회도치.결과 아매소급약후,전자현미경하관찰도실험측삼차신경절세포형태개변,포내출현대량공포,신경섬유수초발생변성、만곡、증후、분리;실험측삼차신경미측척속핵내(CNV)P물질(SP)면역양성과립회도치명현고우대조측(P<0.01),불동시간조지간SP함량차이무통계학의의.결론 광하신경속주사아매소능구선택성파배상응적절세포,감소CNV구SP함량.SP작위일충중요적신경체질,기합성혹석방이상가능여삼차신경통적발병유관,기재삼차신경통적발작궤제중구유중요적작용.
Objective To observe the influence of adriamycin applied locally to infraorbital nerve on the substance P in the lateral nucleus caudalis spinalis nervi trigemini in adult rats. Methods 0. 5% adriamycin was directly injected into the infraor- bital nerve on one side, and normal saline in the opposite as control. The trigeminal ganglions were observed under electro - micro- scope at 3,7,14,21,28,42 days respectively after local administration of adriamycin and normal saline in both groups. Substance P (SP) like positive immunoreactive granules in the lateral nucleus caudalis spinalis nervi trigemini (CNV) on both sides were examined by immunohistochemical method. Results Degenerated trigeminal ganglion ceils were found in adriamycin treated nerves,while myelinated fibers remained intact. The grey scale value of SP like positive immunoreactive granules in the experi- mental side was markedly less than that on control side (P < 0. 01), while no significant difference was present between the groups in different duration. Conclusion Adriamycin locally administered can produce selective destruction of trigeminal gangli- on cells,and can reduce the levels of SP in regions of CNV. As an important neurotransimitter,anbstance P,its synthesis and re- lease is possibly related to the pathogenesis of trigeminsl neuralgia and plays significant role in its onset. The results suggested that local administration of adriamycin is of promising therapeutic implications, further to lay a relevant theoretical foundation in clini- cal management of trigeminal neuralgia.