中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2009年
7期
625-630
,共6页
严良%Joachim S.C.%Pfeiffer N.%Gins F.H.%Erb C.
嚴良%Joachim S.C.%Pfeiffer N.%Gins F.H.%Erb C.
엄량%Joachim S.C.%Pfeiffer N.%Gins F.H.%Erb C.
青光眼,开角型%自身免疫%自身抗体%光谱法,质量,基质辅助激光解吸电离
青光眼,開角型%自身免疫%自身抗體%光譜法,質量,基質輔助激光解吸電離
청광안,개각형%자신면역%자신항체%광보법,질량,기질보조격광해흡전리
Glaucoma open-angle%Autoimmunity%Autoantibodies%Spectrometry,mass,matrix-assis ted laser desorption-ionization
目的 比较原发性开角型青光眼(POAG)患者与正常对照(CTRL)者间的抗体轮廓差异.方法 病例对照研究.将44例POAG患者与48例CTRL者的血清分别加入到含有预洗Dyna珠-G蛋白的反应管中,以捕获人免疫球蛋白G(IgG)抗体,形成的IgG-G蛋白复合体用来捕获目标抗原(匀质化牛视网膜组织).经洗提后获得的抗原蛋白通过"表面增强激光解吸附-电离-飞行时间-质谱"仪进行检测.采用Proteomic software project统计学软件,对两组质谱数据连同受检者年龄和性别等参数,自动进行t检验和初步多变量判别分析;随后采用Statistica version 8.0统计学软件,进行包括抗原蛋白质谱数据、蛋白质芯片类型和激光解吸附-电离能量等的多变量判别分析.结果 CTRL组的平均质谱强度为4244.427±4721.115,POAG组为4049.864±4083.819,两组间差异有统计学意义(F=1.340,P<0.01);在POAG患者中,出现了部分高调节免疫反应,但主要表现为低调节免疫反应.结论 在POAG患者与CTRL者之间存在明显的抗体轮廓差异,在POAG组中出现的低调节免疫反应表明部分患者可能与"异常免疫"有关.(中华眼科杂志,2009,45:625-630)
目的 比較原髮性開角型青光眼(POAG)患者與正常對照(CTRL)者間的抗體輪廓差異.方法 病例對照研究.將44例POAG患者與48例CTRL者的血清分彆加入到含有預洗Dyna珠-G蛋白的反應管中,以捕穫人免疫毬蛋白G(IgG)抗體,形成的IgG-G蛋白複閤體用來捕穫目標抗原(勻質化牛視網膜組織).經洗提後穫得的抗原蛋白通過"錶麵增彊激光解吸附-電離-飛行時間-質譜"儀進行檢測.採用Proteomic software project統計學軟件,對兩組質譜數據連同受檢者年齡和性彆等參數,自動進行t檢驗和初步多變量判彆分析;隨後採用Statistica version 8.0統計學軟件,進行包括抗原蛋白質譜數據、蛋白質芯片類型和激光解吸附-電離能量等的多變量判彆分析.結果 CTRL組的平均質譜彊度為4244.427±4721.115,POAG組為4049.864±4083.819,兩組間差異有統計學意義(F=1.340,P<0.01);在POAG患者中,齣現瞭部分高調節免疫反應,但主要錶現為低調節免疫反應.結論 在POAG患者與CTRL者之間存在明顯的抗體輪廓差異,在POAG組中齣現的低調節免疫反應錶明部分患者可能與"異常免疫"有關.(中華眼科雜誌,2009,45:625-630)
목적 비교원발성개각형청광안(POAG)환자여정상대조(CTRL)자간적항체륜곽차이.방법 병례대조연구.장44례POAG환자여48례CTRL자적혈청분별가입도함유예세Dyna주-G단백적반응관중,이포획인면역구단백G(IgG)항체,형성적IgG-G단백복합체용래포획목표항원(균질화우시망막조직).경세제후획득적항원단백통과"표면증강격광해흡부-전리-비행시간-질보"의진행검측.채용Proteomic software project통계학연건,대량조질보수거련동수검자년령화성별등삼수,자동진행t검험화초보다변량판별분석;수후채용Statistica version 8.0통계학연건,진행포괄항원단백질보수거、단백질심편류형화격광해흡부-전리능량등적다변량판별분석.결과 CTRL조적평균질보강도위4244.427±4721.115,POAG조위4049.864±4083.819,량조간차이유통계학의의(F=1.340,P<0.01);재POAG환자중,출현료부분고조절면역반응,단주요표현위저조절면역반응.결론 재POAG환자여CTRL자지간존재명현적항체륜곽차이,재POAG조중출현적저조절면역반응표명부분환자가능여"이상면역"유관.(중화안과잡지,2009,45:625-630)
Objective To compare the difference of the antibody profile between the primary open angle glaucoma (POAG) and the healthy controls (CTRL). Methods It was a case-control study. Sera from 44 POAG and 48 CTRL were added to tubes containing pre-washed Protein G beads, which can capture IgGs. These IgG-Protein G-complexes were used to capture target antigens (homogenized bovine retina). After elution, the antigenic proteins were measured through surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. By t-test and multivariate statistical analysis, the spectra, the age and the gender between the two groups were compared; subsequently, the antigenic mass spectra data combined with the type of protein chips and the energy of laser desorption/ionization between these two groups were also compared, which indirectly reflect the antibody profiles of these two groups. Results The mean intensity of spectra were 4244.427 ± 4721. 115 in CTRL and 4049.864 ± 4083. 819 in POAG, which showed statistical difference(F = 1. 340, P < 0.01) ; complex antibody profiles were found in both groups. Significantly different immunoreactive spectra were detected between POAG patients and control subjects. Some up-, but mainly down-regulated immunoreactivities were present in POAG patients. Conclusions A significant different pattern of antibody profile is present between the POAG and CTRL. Down-regulated immunoreactivities present in POAG might mean an association between the "aberrant autoimmunity" and the primary open angle glaucoma. (Chin J Ophthalmol , 2009,45:625-630)
