CAJ | 학술논문

建立大鼠输精管平滑肌细胞的培养方法.取大鼠输精管,剥离外膜和内膜,用组织块法进行体外培养.用抗α-SMA(anti α-smooth muscle actin)免疫组化染色的方法鉴定培养的细胞.结果显示,在倒置显微镜下观察细胞形态多样,表现为长梭形或星形,细胞伸出突起互相接触,彼此融合,部分区域细胞多层重叠,部分区域细胞单层高低起伏,呈"峰-谷"状生长.免疫组化染色鉴定呈阳性反应,用该方法所分离、培养的输精管平滑肌细胞纯度达99%以上.应用组织块法培养大鼠输精管-甲滑肌细胞,操作简单,结果稳定.
건립대서수정관평활기세포적배양방법.취대서수정관,박리외막화내막,용조직괴법진행체외배양.용항α-SMA(anti α-smooth muscle actin)면역조화염색적방법감정배양적세포.결과현시,재도치현미경하관찰세포형태다양,표현위장사형혹성형,세포신출돌기호상접촉,피차융합,부분구역세포다층중첩,부분구역세포단층고저기복,정"봉-곡"상생장.면역조화염색감정정양성반응,용해방법소분리、배양적수정관평활기세포순도체99%이상.응용조직괴법배양대서수정관-갑활기세포,조작간단,결과은정.
The aim of this study was to establish a method of isolating and culturing smooth muscle cells from the ductus deferens of rats. Smooth muscle cells were prepared from ductus deferens by explanting technique after dissection of adventitia and intimae, and cultured in vitro. The identification of the smooth muscle cells were verified by using anti a-smooth muscle actin (α-SMA) immunohistochemistry studies. The result suggested that the cells arc multi-morphous, showing long fusiform or star shapes. The apophysis of cells contacted and coalesced to each other, in some regions the cells overlapped in multilayer, while in the other regions they formed monolayer that fluctuated and showed a "peak-valley" shape. They presented a positive reaction through immunohistochemistry studies. The purity of the cells was more than 99% through this method. The culturing of smooth muscle cells by explanting technique is simple and stable.