CAJ | 학술논문

目的探讨慢性乙肝病毒感染者外周血树突状细胞(DCs)亚群[髓系DC(MDC)和浆细胞DC(PDC)]的变化与血清HBVDNA的关系.方法采集健康人和慢性乙肝病毒感染者外周静脉抗凝全血,利用荧光抗体标记和流式细胞仪检测外周血树突状细胞亚群(MDC的特异性标记为Lineage-HLA-DR+CD11c+,PDC的特异性标记为Lineage-HLA-DR+CD123+);固相放免法定量检测血清乙型肝炎病毒标志.结果慢性乙肝病毒感染者外周血PDC相对平均百分率低于健康对照者,但缺少统计学意义,MDC相对数量在两者间没有差别;血清HBeAg阳性慢性乙型肝炎患者外周血PDC相对数量显著高(P<0.01)于HBeAg阴性患者和健康人(P<0.05),而在上述3组中MDC相对数量无差异(P=0.194).结论 HBV感染可导致患者的免疫功能改变,使机体产生免疫耐受或DC的凋亡;慢性乙型肝炎患者外周血PDC增加与HBeAg血清转换有关,提示PDC可能有抑制病毒的作用.
목적 탐토만성을간병독감염자외주혈수돌상세포(DCs)아군[수계DC(MDC)화장세포DC(PDC)]적변화여혈청HBVDNA적관계.방법 채집건강인화만성을간병독감염자외주정맥항응전혈,이용형광항체표기화류식세포의검측외주혈수돌상세포아군(MDC적특이성표기위Lineage-HLA-DR+CD11c+,PDC적특이성표기위Lineage-HLA-DR+CD123+);고상방면법정량검측혈청을형간염병독표지.결과 만성을간병독감염자외주혈PDC상대평균백분솔저우건강대조자,단결소통계학의의,MDC상대수량재량자간몰유차별;혈청HBeAg양성만성을형간염환자외주혈PDC상대수량현저고(P<0.01)우HBeAg음성환자화건강인(P<0.05),이재상술3조중MDC상대수량무차이(P=0.194).결론 HBV감염가도치환자적면역공능개변,사궤체산생면역내수혹DC적조망;만성을형간염환자외주혈PDC증가여HBeAg혈청전환유관,제시PDC가능유억제병독적작용.
Objective To investigate the influence of serum HBVDNA levels on the characterizathion of circulating dendritic cell subsets in patients with chronic hepatitis B infection.Methods The plasmacytoid DC(DC2, Lineage-HLA-DR+CD123+) and non-IFN-alpha producingmyeloid DC (DC1, Lineage-HLA-DR+CD11c+) in fresh peripheral blood from chronic hepatitis B infection without any anti-viral treatment and healthy donors were enumerated by using four-color flow cytometry. The serological markers of hepatitis B virus were evaluated by solid phase radioimmunoassay (RIA).Results Percentage of PDC in patientswith chronic hepatitis B was numerically decreased and subsequently Produce less than that of nomal controls, but without statistical difference. Further, serum HBeAg positive patients with chronic hepatitis B exhibited a significant increase in percentage of circulating PDC, compared with HBeAg negative patients and health donors (P<0.01; P<0.05, respectively). And there is no significant difference of MDC in peripheral blood between chronic hepatitis B patients and nomal controls or among three groups above (P=0.194).Conclusion The results suggested that the HBV infection may cause patient's immunity function change and lead to the immune tolerance or apoptosis ;The percentage of peripheral PDC is associated with the HBeAg seroconversion and may plays a role on inhibition of HBV replication in patients with chronic hepatitis B.