中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2009年
12期
1206-1209
,共4页
薛峰%栾军%张小荣%祝长青%徐飞%陈国强%张常印%徐帮兴%蒋原%陆承平
薛峰%欒軍%張小榮%祝長青%徐飛%陳國彊%張常印%徐幫興%蔣原%陸承平
설봉%란군%장소영%축장청%서비%진국강%장상인%서방흥%장원%륙승평
空肠弯曲菌%多重PCR%API 弯曲菌鉴定系统%流行病学分析%药敏试验
空腸彎麯菌%多重PCR%API 彎麯菌鑒定繫統%流行病學分析%藥敏試驗
공장만곡균%다중PCR%API 만곡균감정계통%류행병학분석%약민시험
Campylobacter jejuni%multi-plex PCR%API campy system%epidemiological investigation%antibiotic resistance test
目的 检测2006-2008年江苏地区鸡、水禽、奶牛、丹顶鹤、猪、实验猴等标本感染空肠弯曲菌及其耐药性.方法 采用APICampy System进行生化与分类鉴定,结合弯曲菌多重PCR检测方法对来自江苏地区的禽、牛、猪、猴3 010份标本,检测分离株及其耐药性.结果 共检测样本3010份, 402份空肠弯曲菌阳性,阳性率13.36%,其中家禽样阳性率15.83%(258/1630);水禽10.40%(52/500);奶牛7.65 %(39/510);猪18.75%(30/160);猴15.00%(15/100);丹顶鹤12.50%(10/80);糜鹿0%(0/30).选择不同宿主来源的108株对10大类27种抗生素高度敏感率的是: 氯霉素100%、阿莫西林99.07%、阿米卡星92.59%、头孢噻肟91.67%、阿齐霉素90.74%;高度耐药率的是: 头孢哌酮99.07%、复方新诺明99.07%、头孢孟多99.07%、磺胺甲基异噁唑97.22%、头孢拉丁91.67 %.结果 显示,108株分离株的耐药主要集中在9耐~20耐,占85.19% ,猪分离株的多重耐药性较其它宿主来源分离株严重.结论 江苏地区空肠弯曲菌的流行和耐药状况呈现多样化和复杂化, 不同宿主来源分离株耐药性和多重耐药性存在差异.
目的 檢測2006-2008年江囌地區鷄、水禽、奶牛、丹頂鶴、豬、實驗猴等標本感染空腸彎麯菌及其耐藥性.方法 採用APICampy System進行生化與分類鑒定,結閤彎麯菌多重PCR檢測方法對來自江囌地區的禽、牛、豬、猴3 010份標本,檢測分離株及其耐藥性.結果 共檢測樣本3010份, 402份空腸彎麯菌暘性,暘性率13.36%,其中傢禽樣暘性率15.83%(258/1630);水禽10.40%(52/500);奶牛7.65 %(39/510);豬18.75%(30/160);猴15.00%(15/100);丹頂鶴12.50%(10/80);糜鹿0%(0/30).選擇不同宿主來源的108株對10大類27種抗生素高度敏感率的是: 氯黴素100%、阿莫西林99.07%、阿米卡星92.59%、頭孢噻肟91.67%、阿齊黴素90.74%;高度耐藥率的是: 頭孢哌酮99.07%、複方新諾明99.07%、頭孢孟多99.07%、磺胺甲基異噁唑97.22%、頭孢拉丁91.67 %.結果 顯示,108株分離株的耐藥主要集中在9耐~20耐,佔85.19% ,豬分離株的多重耐藥性較其它宿主來源分離株嚴重.結論 江囌地區空腸彎麯菌的流行和耐藥狀況呈現多樣化和複雜化, 不同宿主來源分離株耐藥性和多重耐藥性存在差異.
목적 검측2006-2008년강소지구계、수금、내우、단정학、저、실험후등표본감염공장만곡균급기내약성.방법 채용APICampy System진행생화여분류감정,결합만곡균다중PCR검측방법대래자강소지구적금、우、저、후3 010빈표본,검측분리주급기내약성.결과 공검측양본3010빈, 402빈공장만곡균양성,양성솔13.36%,기중가금양양성솔15.83%(258/1630);수금10.40%(52/500);내우7.65 %(39/510);저18.75%(30/160);후15.00%(15/100);단정학12.50%(10/80);미록0%(0/30).선택불동숙주래원적108주대10대류27충항생소고도민감솔적시: 록매소100%、아막서림99.07%、아미잡성92.59%、두포새우91.67%、아제매소90.74%;고도내약솔적시: 두포고동99.07%、복방신낙명99.07%、두포맹다99.07%、광알갑기이오서97.22%、두포랍정91.67 %.결과 현시,108주분리주적내약주요집중재9내~20내,점85.19% ,저분리주적다중내약성교기타숙주래원분리주엄중.결론 강소지구공장만곡균적류행화내약상황정현다양화화복잡화, 불동숙주래원분리주내약성화다중내약성존재차이.
From 2006 to 2008, the susceptibility of different species of animal hosts to Campyebacter jejubni infection was observed in various areas of Jiangsu province, in which the API Campy System was used to perform the biochemical identification and the multiple PCR assay was employed to analyse the C.jejuni isolates from 3010 specimens of fouls. Cattle, pigs and monkeys, and in addition, the susceptibility of isolates to various antibiotics was also determined. In these specimens investigated 402 samples were found to be positive in the detection of C.jejuni with a positive detection rate of 13.36%. The positive detection rates in chicken, water fouls, milk cows, pigs, monkey, red crowned crane and wapiti were 15.83% (258/1630), 10.4% (52/100), 8.24% (42/510), 15.63% (25/160), 15% (15/100), 12.5% (15/80) and 0% (0/30) respectively. Meanwhile, the antibiotics to which the isolates from different hosts showed high rate of sensitivity to 27 antibiotics of 10 varieties included: chloromycetin (100%), Almocylin (99.7%), amicarcin (92.59%), cefprozil (91.67%), alchimycin (90.74%); while the antibiotics to which these isolates showed high rate of resistance were compound neoromin (99.7%), cefoperazone (99.07%), trimethoprim (97.22%), cepronatin (91.67%), cepromondo (99,07%) respectively. It is evident that the susceptibility of different hosts to C.jejuni infection and the status of drug-resistance of the isolates appear to be quite different and more complicated in Jiangsu province.
